1H, 13C and 15N backbone resonance assignment of the calcium-activated EndoU endoribonuclease

IF 0.8 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2024-09-09 DOI:10.1007/s12104-024-10198-y

Florian Malard, Fedor V. Karginov, Sébastien Campagne

引用次数: 0

Abstract

The catalytic domain of the calcium-dependent endoribonuclease EndoU from Homo sapiens was expressed in E. coli with ¹³C and ¹⁵N labeling. A nearly complete assignment of backbone ¹H, ¹⁵N, and ¹³C resonances was obtained, as well as a secondary structure prediction based on the assigned chemical shifts. The predicted secondary structures were almost identical to the published crystal structure of calcium-activated EndoU. This is the first NMR study of an eukaryotic member of the EndoU-like superfamily of ribonucleases.

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钙激活的 EndoU 内切核酸酶的 1H、13C 和 15N 主干共振分配。

用 13C 和 15N 标记在大肠杆菌中表达了智人的钙依赖性内切核酸酶 EndoU 的催化结构域。对骨架 1H、15N 和 13C 共振进行了近乎完整的分配，并根据分配的化学位移预测了二级结构。预测的二级结构与已发表的钙激活 EndoU 晶体结构几乎完全相同。这是首次对核糖核酸酶超家族中类似 EndoU 的真核生物成员进行核磁共振研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.