Revised version with tracked changes oral Magnesium reduces levels of pathogenic autoantibodies and skin disease in murine lupus.

IF 2.9 4区 医学 Q3 IMMUNOLOGY
Alberto Verlato, Teresina Laragione, Sofia Bin, Randie H Kim, Fadi Salem, Percio S Gulko, Paolo Cravedi
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Abstract

Background: Systemic Lupus Erythematosus (SLE) has a strong genetic susceptibility, but little is known about the impact of diet on disease severity. The Western diet is typically deficient in magnesium (Mg), and given the immunomodulatory effects of Mg, we hypothesized that the low Mg intake increases disease risk and that increasing Mg intake would reduce severity of murine lupus. Here, we placed 12-week old MRL/lpr female lupus mice on a normal (Mg500) or a high (Mg2800) Mg diet for 9 weeks. Urine and blood were collected during the study for quantification of urinary albumin, BUN, anti-dsDNA antibodies, and immune phenotyping.

Results: MRL/lpr lupus mice on high Mg2800 diet had significantly fewer skin lesions and less severe skin histology score, and reduced levels of pathogenic anti-dsDNA antibodies, compared with the Mg500 group (143.8±75.0 vs. 47.4±36.2 × 106U/ml; P < 0.05). The high Mg2800 group had a nearly two-fold increase in the percentage of CD4+FOXP3+ Treg cells compared to controls (19.9±5.4 vs. 11.4±5.5%; P < 0.05). Treg percentages inversely correlated with the concentration of anti-dsDNA. None of the mice developed arthritis during the observation period and there were no significant differences in weight, proteinuria, BUN or kidney histology.

Conclusion: In conclusion, oral supplementation of Mg has a protective effect in a murine lupus model and may represent an inexpensive and safe adjuvant in the treatment of SLsE.

修订版口服镁可降低小鼠狼疮的致病性自身抗体水平和皮肤病。
背景:系统性红斑狼疮(SLE)有很强的遗传易感性,但人们对饮食对疾病严重程度的影响知之甚少。西方饮食中通常缺乏镁(Mg),鉴于镁具有免疫调节作用,我们假设镁摄入量低会增加患病风险,而增加镁摄入量会降低小鼠狼疮的严重程度。在这里,我们将 12 周大的 MRL/lpr 雌性狼疮小鼠置于正常(Mg500)或高(Mg2800)镁饮食中 9 周。研究期间收集尿液和血液,以定量检测尿白蛋白、BUN、抗dsDNA抗体和免疫表型:结果:与 Mg500 组(143.8±75.0 vs. 47.4±36.2 × 106U/ml;P +FOXP3+ Treg 细胞与对照组相比(19.9±5.4 vs. 11.4±5.5%;P)相比,MRL/lpr 狼疮小鼠摄入高 Mg2800 食物后,皮肤病变显著减少,皮肤组织学评分较轻,致病性抗dsDNA 抗体水平降低:总之,口服补充镁对小鼠狼疮模型有保护作用,可能是治疗 SLsE 的一种廉价、安全的辅助疗法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Immunology
BMC Immunology 医学-免疫学
CiteScore
5.50
自引率
0.00%
发文量
54
审稿时长
1 months
期刊介绍: BMC Immunology is an open access journal publishing original peer-reviewed research articles in molecular, cellular, tissue-level, organismal, functional, and developmental aspects of the immune system as well as clinical studies and animal models of human diseases.
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