Flynotyper 2.0: An updated tool for rapid quantitative assessment of Drosophila eye phenotypes.

IF 2.1 3区 生物学 Q3 GENETICS & HEREDITY
Johnathan Ray, Deepro Banerjee, Qingyu Wang, Santhosh Girirajan
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引用次数: 0

Abstract

About two-thirds of the genes in the Drosophila melanogaster genome are also involved in its eye development, making the Drosophila eye an ideal system for genetic studies. We previously developed Flynotyper, a software that uses image processing operations to identify and quantify the degree of roughness by measuring disorderliness of ommatidial arrangement in the fly eye. This software has enabled researchers to quantify morphological defects of thousands of eye images caused by genetic perturbations. Here, we present Flynotyper 2.0, a software that has an updated computer vision library, improved performance, and a streamlined pipeline for high-throughput analysis of multiple eye images. We also tested several batches of Drosophila eye images to ensure robustness and reproducibility of the updated Flynotyper 2.0 software.

Flynotyper 2.0:果蝇眼睛表型快速定量评估的最新工具。
黑腹果蝇基因组中约有三分之二的基因也参与了眼睛的发育,因此果蝇眼睛是遗传研究的理想系统。我们之前开发了 Flynotyper 软件,该软件利用图像处理操作,通过测量果蝇眼球中膜排列的无序性来识别和量化粗糙程度。通过该软件,研究人员可以量化数千个眼睛图像中由遗传扰动引起的形态缺陷。在此,我们介绍 Flynotyper 2.0,该软件更新了计算机视觉库,提高了性能,并简化了对多幅眼睛图像进行高通量分析的流程。我们还测试了几批果蝇眼睛图像,以确保更新后的 Flynotyper 2.0 软件的稳健性和可重复性。
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来源期刊
G3: Genes|Genomes|Genetics
G3: Genes|Genomes|Genetics GENETICS & HEREDITY-
CiteScore
5.10
自引率
3.80%
发文量
305
审稿时长
3-8 weeks
期刊介绍: G3: Genes, Genomes, Genetics provides a forum for the publication of high‐quality foundational research, particularly research that generates useful genetic and genomic information such as genome maps, single gene studies, genome‐wide association and QTL studies, as well as genome reports, mutant screens, and advances in methods and technology. The Editorial Board of G3 believes that rapid dissemination of these data is the necessary foundation for analysis that leads to mechanistic insights. G3, published by the Genetics Society of America, meets the critical and growing need of the genetics community for rapid review and publication of important results in all areas of genetics. G3 offers the opportunity to publish the puzzling finding or to present unpublished results that may not have been submitted for review and publication due to a perceived lack of a potential high-impact finding. G3 has earned the DOAJ Seal, which is a mark of certification for open access journals, awarded by DOAJ to journals that achieve a high level of openness, adhere to Best Practice and high publishing standards.
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