Alteration of lncRNA RHPN1-AS1 predicts clinical prognosis and regulates the progression of bladder cancer via modulating miR-485-5p.

IF 2.3 4区 医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jingmin Zhou, Jinshan Xu, Lin Cheng, Shuhui Li, Deqi Jiang, Jianchao Zhang, Yulong Sheng
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引用次数: 0

Abstract

Background: Exploring effect biomarkers that monitor tumor progression and predict the prognosis could benefit the clinical management of bladder cancer and improve the postoperative life of patients. This study aimed to estimate the function of long non-coding (lnc)RNA RHPN1-AS1 (RHPN1-AS1) in bladder cancer and the potential molecular mechanism.

Methods: The expression of RHPN1-AS1 was evaluated in bladder cancer tissues from 115 patients and cells by polymerase chain reaction. The clinical significance of RHPN1-AS1 was assessed and its effect was also estimated in cell proliferation, migration, and invasion. The underlying molecular mechanism was explored by the dual-luciferase reporter assay.

Results: The expression of RHPN1-AS1 was 2.91-fold elevated in bladder cancer, which showed a close correlation with advanced tumor node metastasis stage (P = 0.013) and the presence of lymph node metastasis (P = 0.018). RHPN1-AS1 also served as a poor prognostic indicator (hazard ratio = 2.563) for bladder cancer. The knockdown of RHPN1-AS1 significantly suppressed the proliferation and metastasis ability of bladder cancer cells. Moreover, miR-485-5p was found to mediate the function of RHPN1-AS1 in bladder cancer, which was considered the underlying regulatory mechanism.

Conclusions: RHPN1-AS1 serves as a prognostic biomarker and tumor promoter in bladder cancer via modulating miR-485-5p, which might be a reliable target of bladder cancer therapy.

lncRNA RHPN1-AS1 的改变可预测临床预后,并通过调节 miR-485-5p 来调控膀胱癌的进展。
背景:探索监测肿瘤进展和预测预后的效应生物标志物将有利于膀胱癌的临床治疗并改善患者的术后生活。本研究旨在估测长非编码(lnc)RNA RHPN1-AS1(RHPN1-AS1)在膀胱癌中的功能及其潜在的分子机制:方法:通过聚合酶链反应评估RHPN1-AS1在115例膀胱癌患者组织和细胞中的表达。评估了 RHPN1-AS1 的临床意义,并估计了其对细胞增殖、迁移和侵袭的影响。结果发现,RHPN1-AS1的表达与膀胱癌细胞的增殖、迁移和侵袭有关:结果:RHPN1-AS1在膀胱癌中的表达量升高了2.91倍,与晚期肿瘤结节转移分期(P = 0.013)和淋巴结转移(P = 0.018)密切相关。RHPN1-AS1 还是膀胱癌的不良预后指标(危险比 = 2.563)。敲除 RHPN1-AS1 能显著抑制膀胱癌细胞的增殖和转移能力。此外,研究还发现miR-485-5p介导了RHPN1-AS1在膀胱癌中的功能,这被认为是潜在的调控机制:结论:RHPN1-AS1通过调节miR-485-5p成为膀胱癌的预后生物标志物和肿瘤促进因子,可能成为膀胱癌治疗的可靠靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
International Journal of Biological Markers
International Journal of Biological Markers 医学-生物工程与应用微生物
CiteScore
4.10
自引率
0.00%
发文量
43
期刊介绍: IJBM is an international, online only, peer-reviewed Journal, which publishes original research and critical reviews primarily focused on cancer biomarkers. IJBM targets advanced topics regarding the application of biomarkers in oncology and is dedicated to solid tumors in adult subjects. The clinical scenarios of interests are screening and early diagnosis of cancer, prognostic assessment, prediction of the response to and monitoring of treatment.
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