Comparative analysis of biological potential of acerola by-product extracts obtained by hydrothermal and gas-expanded liquid extraction

IF 3.4 3区工程技术 Q2 CHEMISTRY, PHYSICAL

Journal of Supercritical Fluids Pub Date : 2024-08-31 DOI:10.1016/j.supflu.2024.106394

Otília Mônica Alves Borges , Karina Cesca , Laís Benvenutti , Matheus Henrique Oliveira de Sousa , Neusa Fernandes de Moura , Jose David Sánchez-Martinez , Alejandro Cifuentes , Acácio A.F. Zielinski , Patrícia Poletto

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Abstract

The acerola non-pomace, a by-product from the juice clarification step and rich in phenolic compounds and ascorbic acid, was investigated for its biological potential through in vitro tests. Extracts were obtained using hydrothermal extraction (HT) at 121°C as well as CO₂-expanded ethanol (GXL) at 40°C. The GXL extract contained 159 mg/g of ascorbic acid, while the HT extract showed 70 mg/g. Catechin was found only in the HT extract, and quercetin only in the GXL extract. Both extracts exhibited similar antiglycemic potential, but the GXL extract showed twice the neuroprotective potential against acetylcholinesterase (AChE, IC₅₀=302 μg/mL) and lipoxygenase (LOX, IC₅₀=227 μg/mL) activities. In human breast cancer cells (MDA-MB-231), the GXL extract demonstrated better antitumor potential with an IC₅₀ of 100 μg/mL compared to 250 μg/mL for the HT extract.

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通过水热萃取和气体膨胀液体萃取获得的针叶树副产品提取物的生物潜力比较分析

金针菜非渣是果汁澄清步骤中的副产品，富含酚类化合物和抗坏血酸，研究人员通过体外试验对其生物潜力进行了研究。采用 121°C 水热萃取（HT）和 40°C 二氧化碳膨胀乙醇（GXL）提取物。GXL 提取物含有 159 毫克/克的抗坏血酸，而 HT 提取物则为 70 毫克/克。儿茶素只存在于 HT 提取物中，而槲皮素只存在于 GXL 提取物中。两种提取物都具有类似的降血糖潜力，但 GXL 提取物对乙酰胆碱酯酶（AChE，IC50=302 μg/mL）和脂氧合酶（LOX，IC50=227 μg/mL）活性的神经保护潜力是后者的两倍。在人类乳腺癌细胞（MDA-MB-231）中，GXL 提取物显示出更好的抗肿瘤潜力，其 IC50 为 100 μg/mL，而 HT 提取物的 IC50 为 250 μg/mL。

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来源期刊

Journal of Supercritical Fluids 工程技术-工程：化工

CiteScore

7.60

自引率

10.30%

发文量

236

审稿时长

56 days

期刊介绍： The Journal of Supercritical Fluids is an international journal devoted to the fundamental and applied aspects of supercritical fluids and processes. Its aim is to provide a focused platform for academic and industrial researchers to report their findings and to have ready access to the advances in this rapidly growing field. Its coverage is multidisciplinary and includes both basic and applied topics. Thermodynamics and phase equilibria, reaction kinetics and rate processes, thermal and transport properties, and all topics related to processing such as separations (extraction, fractionation, purification, chromatography) nucleation and impregnation are within the scope. Accounts of specific engineering applications such as those encountered in food, fuel, natural products, minerals, pharmaceuticals and polymer industries are included. Topics related to high pressure equipment design, analytical techniques, sensors, and process control methodologies are also within the scope of the journal.