{"title":"Comparative analysis of biological potential of acerola by-product extracts obtained by hydrothermal and gas-expanded liquid extraction","authors":"","doi":"10.1016/j.supflu.2024.106394","DOIUrl":null,"url":null,"abstract":"<div><p>The acerola non-pomace, a by-product from the juice clarification step and rich in phenolic compounds and ascorbic acid, was investigated for its biological potential through <em>in vitro</em> tests. Extracts were obtained using hydrothermal extraction (HT) at 121°C as well as CO<sub>2</sub>-expanded ethanol (GXL) at 40°C. The GXL extract contained 159 mg/g of ascorbic acid, while the HT extract showed 70 mg/g. Catechin was found only in the HT extract, and quercetin only in the GXL extract. Both extracts exhibited similar antiglycemic potential, but the GXL extract showed twice the neuroprotective potential against acetylcholinesterase (AChE, IC<sub>50</sub>=302 μg/mL) and lipoxygenase (LOX, IC<sub>50</sub>=227 μg/mL) activities. In human breast cancer cells (MDA-MB-231), the GXL extract demonstrated better antitumor potential with an IC<sub>50</sub> of 100 μg/mL compared to 250 μg/mL for the HT extract.</p></div>","PeriodicalId":17078,"journal":{"name":"Journal of Supercritical Fluids","volume":null,"pages":null},"PeriodicalIF":3.4000,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Supercritical Fluids","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0896844624002298","RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}
引用次数: 0
Abstract
The acerola non-pomace, a by-product from the juice clarification step and rich in phenolic compounds and ascorbic acid, was investigated for its biological potential through in vitro tests. Extracts were obtained using hydrothermal extraction (HT) at 121°C as well as CO2-expanded ethanol (GXL) at 40°C. The GXL extract contained 159 mg/g of ascorbic acid, while the HT extract showed 70 mg/g. Catechin was found only in the HT extract, and quercetin only in the GXL extract. Both extracts exhibited similar antiglycemic potential, but the GXL extract showed twice the neuroprotective potential against acetylcholinesterase (AChE, IC50=302 μg/mL) and lipoxygenase (LOX, IC50=227 μg/mL) activities. In human breast cancer cells (MDA-MB-231), the GXL extract demonstrated better antitumor potential with an IC50 of 100 μg/mL compared to 250 μg/mL for the HT extract.
期刊介绍：
The Journal of Supercritical Fluids is an international journal devoted to the fundamental and applied aspects of supercritical fluids and processes. Its aim is to provide a focused platform for academic and industrial researchers to report their findings and to have ready access to the advances in this rapidly growing field. Its coverage is multidisciplinary and includes both basic and applied topics.
Thermodynamics and phase equilibria, reaction kinetics and rate processes, thermal and transport properties, and all topics related to processing such as separations (extraction, fractionation, purification, chromatography) nucleation and impregnation are within the scope. Accounts of specific engineering applications such as those encountered in food, fuel, natural products, minerals, pharmaceuticals and polymer industries are included. Topics related to high pressure equipment design, analytical techniques, sensors, and process control methodologies are also within the scope of the journal.
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