Study of NALT1 and CTBP1-AS1 lncRNAs expression levels in triple-negative breast cancer patients in the Iranian population

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2024-09-01 DOI:10.1016/j.genrep.2024.102021

Elham Aminirad , Tala Haghnazari-Esfahlan , Ali Rajabi, Elaheh Hassannezhad-Daneshmand, Reza Safaralizadeh

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引用次数: 0

Abstract

Background

Triple-negative breast cancer (TNBC), an aggressive subtype with a poor prognosis, is notably difficult to treat. Emerging research highlights the significant roles of long non-coding RNAs (lncRNAs) in cancer biology. LncRNAs, such as NALT1 and CTBP1-AS1 are implicated in oncogenic processes; this study hypothesizes that NALT1 and CTBP1-AS1 are overexpressed in TNBC tissues compared to adjacent non-tumor tissues and may serve as biomarkers. Methods: One hundred pairs of tumor and adjacent non-tumor tissues were obtained from female patients with triple-negative breast cancer. After extracting RNA, cDNA synthesis was carried out for all samples. Quantitative real-time PCR (qRT-PCR) was employed to assess differential gene expression. Results: The expression of NALT1 (p-value <0.0001) and CTBP1-AS1 (p-value <0.0002) lncRNAs increased in TNBC tumor tissues in comparison to adjacent non-tumor tissues. A statistically positive correlation (ρ = 0.5844, p < 0.0001) was observed between the expression levels of NALT1 and CTBP1-AS1 in breast cancer patients. The ROC analysis indicated that NALT1 (AUC = 0.718, specificity = 61 %, sensitivity = 70 %) shows moderate potential and CTBP1-AS1 (AUC = 0.648, specificity = 65 %, sensitivity = 55 %) exhibits poor potential as a diagnostic biomarker for breast cancer. Conclusion: This study shows that NALT1 and CTBP1-AS1 lncRNAs are upregulated in TNBC tissues. Additionally, a positive correlation exists between their expression levels in breast cancer. Further research is needed to understand their mechanisms as molecular biomarkers.

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伊朗三阴性乳腺癌患者中 NALT1 和 CTBP1-AS1 lncRNAs 表达水平的研究

背景三阴性乳腺癌（TNBC）是一种侵袭性亚型癌症，预后较差，尤其难以治疗。新近的研究强调了长非编码 RNA（lncRNA）在癌症生物学中的重要作用。NALT1和CTBP1-AS1等LncRNA与致癌过程有关；本研究推测，与邻近的非肿瘤组织相比，NALT1和CTBP1-AS1在TNBC组织中过表达，可作为生物标志物。研究方法从女性三阴性乳腺癌患者中获取 100 对肿瘤组织和邻近非肿瘤组织。提取 RNA 后，对所有样本进行 cDNA 合成。采用定量实时 PCR（qRT-PCR）技术评估不同基因的表达。结果显示与邻近的非肿瘤组织相比，NALT1（p-value <0.0001）和CTBP1-AS1（p-value <0.0002）lncRNA在TNBC肿瘤组织中的表达量增加。NALT1和CTBP1-AS1在乳腺癌患者中的表达水平呈统计学正相关（ρ = 0.5844, p <0.0001）。ROC分析表明，NALT1（AUC = 0.718，特异性 = 61 %，灵敏度 = 70 %）显示出中等潜力，而 CTBP1-AS1（AUC = 0.648，特异性 = 65 %，灵敏度 = 55 %）作为乳腺癌诊断生物标记物的潜力较差。结论本研究表明，NALT1 和 CTBP1-AS1 lncRNA 在 TNBC 组织中上调。此外，它们在乳腺癌中的表达水平呈正相关。要了解它们作为分子生物标志物的机制，还需要进一步的研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.