True empty follicle syndrome is a subtype of oocyte maturation abnormalities.

IF 1 Q4 OBSTETRICS & GYNECOLOGY
Şafak Hatırnaz, Ebru Hatırnaz, Justin Tan, Samettin Çelik, Canan Soyer Çalışkan, Alper Başbuğ, Gerçek Aydın, Ali Bahadırlı, Mehmet Bülbül, Handan Çelik, Aşkı Ellibeş Kaya, Nur Dokuzeylül Güngör, Seang Lin Tan, Mingju Cao, Michael H Dahan, Sebati Sinan Ürkmez
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引用次数: 0

Abstract

Objective: To review the outcomes of in vitro maturation (IVM) and in vitro fertilization (IVF) in women with empty follicle syndrome (EFS). The study evaluated the genetic underpinnings of EFS by analyzing mutations.

Materials and methods: This retrospective case series involving 17 women with EFS over at least 2 IVF cycles was conducted. The study also employed whole-exome sequencing to analyze the genetic mutations. The treatment approaches included letrozole-primed IVM, follicle-stimulating hormone (FSH)-human chorionic gonadotrophin (hCG)-primed IVM, and conventional IVF.

Results: The average female age was 31.5±4.6 years, and the duration of infertility was 7.3±3.5 years. Four patients underwent IVF. IVM oocyte collections yielded oocytes in 12 of 13 subjects. Of these, 75% (9/12) yielded MII oocytes after 48 h of IVM media incubation. Six subjects had fertilized embryos, resulting in a 40.9% intracytoplasmic sperm injection (ICSI) fertilization rate (9 embryos/22 MII oocytes). Genetic analysis revealed mutations in seven patients. This study demonstrated the partial efficacy of letrozole-primed IVM plus growth hormone and FSH-hCG primed IVM protocols. No pregnancies or live births were recorded after IVM. One ongoing pregnancy post-IVF and one spontaneous live birth were observed.

Conclusion: Inter-cycle variabilities were observed in women with oocyte maturation abnormalities (OMAs). Almost all patients with EFS had oocytes collected during IVM following IVF. These oocytes have limited potential for maturation, fertilization, and live birth, as demonstrated by the low rates observed after IVM culture and ICSI. These conditions are observed in OMAs due to defects in the oocyte machinery. The proposed flowchart provides a comprehensive classification approach for various forms of EFS.

真性空卵泡综合征是卵母细胞成熟异常的一种亚型。
目的回顾空卵泡综合征(EFS)妇女体外成熟(IVM)和体外受精(IVF)的结果。研究通过分析突变评估了 EFS 的遗传基础:这项回顾性病例系列研究涉及 17 名至少经历过 2 个试管婴儿周期的 EFS 妇女。研究还采用了全外显子组测序来分析基因突变。治疗方法包括来曲唑促排卵试管婴儿、卵泡刺激素(FSH)-人绒毛膜促性腺激素(hCG)促排卵试管婴儿和常规试管婴儿:女性平均年龄为(31.5±4.6)岁,不孕时间为(7.3±3.5)年。四名患者接受了体外受精。在 13 名受试者中,有 12 人进行了 IVM 卵母细胞采集。其中,75%(9/12)的患者在 IVM 培养基培养 48 小时后获得了 MII 卵母细胞。有六名受试者获得了受精胚胎,卵胞浆内单精子显微注射(ICSI)受精率为 40.9%(9 个胚胎/22 个 MII 卵母细胞)。基因分析显示,7 名患者存在基因突变。这项研究证明了来曲唑促排卵 IVM 加生长激素和 FSH-hCG 促排卵 IVM 方案的部分疗效。体外受精后没有怀孕或活产的记录。观察到一次体外受精后的持续妊娠和一次自然活产:结论:在卵母细胞成熟异常(OMAs)妇女中观察到了周期间变异。几乎所有 EFS 患者都是在体外受精后的 IVM 期间采集的卵母细胞。这些卵母细胞在成熟、受精和活产方面的潜力有限,IVM 培养和 ICSI 后观察到的低成功率就证明了这一点。在 OMA 中观察到的这些情况是由于卵母细胞机制的缺陷造成的。拟议的流程图为各种形式的 EFS 提供了一种全面的分类方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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CiteScore
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