Influence of exogenous 24-epibrassinolide on improving carotenoid content, antioxidant capacity and gene expression in germinated maize seeds.

IF 3.3 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2024-09-04 DOI:10.1002/jsfa.13870

Wenying Yang, Hao Xu, Fanyu Wang, Weiwei He, Dajing Li, Qingqi Guo, Yihong Bao, Zhongyuan Zhang

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引用次数: 0

Abstract

Background: Carotenoids have various physiological functions, such as immune regulation and cancer prevention. Germination could further improve the content of carotenoids in maize seeds. In this study, yellow maize seeds (Suyu 29) were soaked and germinated with different concentrations of 24-epibrassinolide. The changes of germination percentage, sprout length, bioactive components, antioxidant capacity and carotenoid content of the maize seeds were analyzed. Additionally, the relative expression of key genes in the carotenoid synthesis pathway was investigated.

Results: The results showed that the sprout length, germination percentage, soluble protein, free amino acids, proline, endogenous abscisic acid, vitamin C, total phenolics and carotenoids displayed a significant increasing trend compared with the control group (P < 0.05). The activity of superoxide dismutase and peroxidase increased by 55.1% and 58.5% versus the control group, and the antioxidant capacity of 2,2-diphenyl-1-picrylhydrazyl, 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) and ferric reducing antioxidant power was 19.8%, 13.4% and 44.1% higher than that of the control group (P < 0.05). Compared with the control group, the expression of genes was significantly up-regulated (P < 0.05). Under the treatment of 0.1 mg L^-1 of 24-epibrassinolide, carotenoid content reached the highest value. The carotenoids showed a positive correspondence with antioxidant enzyme activity, antioxidant capacity and total phenolics content (P < 0.05).

Conclusion: This study showed that 0.1 mg L^-1 of exogenous 24-epibrassinolide promoted the accumulation of carotenoids and improved the antioxidant capacity and the quality of germinated maize seeds. It could provide a method for the development of germinated maize products enriched in carotenoids. © 2024 Society of Chemical Industry.

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外源 24-epibrassinolide 对提高发芽玉米种子类胡萝卜素含量、抗氧化能力和基因表达的影响

背景：类胡萝卜素具有多种生理功能，如免疫调节和癌症预防。催芽可进一步提高玉米种子中类胡萝卜素的含量。本研究用不同浓度的 24-eprassinolide 对黄玉米种子（苏玉 29）进行浸泡和催芽。分析了玉米种子的发芽率、芽长、生物活性成分、抗氧化能力和类胡萝卜素含量的变化。此外，还研究了类胡萝卜素合成途径中关键基因的相对表达：结果表明，与对照组相比，24-表紫苏内酯组的萌发长度、发芽率、可溶性蛋白质、游离氨基酸、脯氨酸、内源赤霉酸、维生素 C、总酚类和类胡萝卜素呈显著增加趋势（P -1），类胡萝卜素含量达到最高值。类胡萝卜素与抗氧化酶活性、抗氧化能力和总酚含量呈正相关（P 结论：类胡萝卜素与抗氧化酶活性、抗氧化能力和总酚含量呈正相关：本研究表明，0.1 mg L-1 的外源 24-epibrassinolide 能促进类胡萝卜素的积累，提高抗氧化能力，改善发芽玉米种子的质量。这为开发富含类胡萝卜素的发芽玉米产品提供了一种方法。© 2024 化学工业协会。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.