Treatment of sewage sludge with γ-ray irradiation for volatile fatty acids and lipid production

Abstract

The production of value-added products from sewage sludge is considered to be one of the solutions for the sustainable management of sludge in wastewater treatment plants (WWTPs). The presence of carbon, nitrogen, and phosphorus sources has made the sewage sludge of WWTPs a valuable and low-cost substrate for the production of fermentative products. In the current study, a process was developed for microbial lipid production from two types of sewage sludge from a WWTP in northern Isfahan: anaerobic digester inlet sludge (DIS) and anaerobic digester outlet sludge (DOS). This process was based on the release of volatile fatty acids (VFAs) from the sludge by combinations of γ-ray irradiation, anaerobic digestion, and acidogenic fermentation followed by utilization of VFAs in a microbial process by the oleaginous yeast Cryptococcus aureus UIMC65. After γ-ray irradiation, the acidogenic fermentation of the treated sludge released 72% of the organic matter content of the sludge with acidification efficiency of 12% leading to 0.516 g L⁻¹ VFAs. The oleaginous fermentation of the released VFAs for 7 days was accompanied by production of 1.58 g L⁻¹ dry cell biomass with 40% lipid content. The results of this study indicate that the sewage sludge from urban WWTP has the potential to be used for the production of microbial lipids.