Analysis of the Dynamic Expression of the SMAD Family in the Periosteum During Guided Bone Regeneration.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-09-02 DOI:10.1097/SCS.0000000000010498

Bao-Fu Yu, Xiao-Qing Li, Xiao-Xue Chen, Chuan-Chang Dai, Jin-Guang He, Jiao Wei

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引用次数: 0

Abstract

Objective: The aim of this study was to investigate the dynamic expression of the SMAD family during guided bone regeneration for the reconstruction of cranio-maxillofacial bone defects.

Methods: A swine model of guided bone regeneration was established with one side of the rib as the trauma group and the contralateral as control group. Periosteal and regenerative tissue specimens were harvested at 9 time points in the early, middle, and late phases, and were subjected to gene sequencing and tissue staining. Expression data of each SMAD family were extracted for further analysis, in which the correlation of the expression of the respective members within and between groups and at different time points was analyzed.

Results: The expression of individual members of the SMAD family fluctuates greatly, especially during the first month. The SMAD3 and SMAD4 genes were the most highly expressed. The foldchange value of SMAD6 was the largest and remained above 1.5 throughout the process. The dynamic expression levels of SMAD2, SMAD4, SMAD5, SMAD6, and SMAD9 showed a significant positive correlation in both groups. The expression levels of each gene showed a positive correlation with other SMAD genes. Tissue staining showed that the overall contour of the regenerated bone tissue was basically formed within the first 1 month.

Conclusion: The first month of guided bone regeneration is a critical period for bone regeneration and is an important period for the SMAD family to play a role. The SMAD6 may play an important role in the whole process of guided bone regeneration.

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骨引导再生过程中 SMAD 家族在骨膜中的动态表达分析

研究目的本研究旨在探讨 SMAD 家族在引导骨再生重建颅颌面骨缺损过程中的动态表达：方法：以一侧肋骨为创伤组，对侧为对照组，建立猪引导骨再生模型。在早期、中期和晚期的 9 个时间点采集骨膜和再生组织标本，并进行基因测序和组织染色。提取每个 SMAD 家族的表达数据进行进一步分析，其中分析了组内、组间和不同时间点各成员表达的相关性：结果：SMAD 家族各成员的表达波动很大，尤其是在第一个月。SMAD3和SMAD4基因的表达量最高。SMAD6 的折叠变化值最大，在整个过程中都保持在 1.5 以上。两组中，SMAD2、SMAD4、SMAD5、SMAD6 和 SMAD9 的动态表达水平呈显著正相关。各基因的表达水平与其他 SMAD 基因呈正相关。组织染色显示，再生骨组织的整体轮廓在最初 1 个月内基本形成：结论：引导骨再生的第一个月是骨再生的关键时期，也是 SMAD 家族发挥作用的重要时期。SMAD6可能在整个引导骨再生过程中发挥重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.