Untargeted metabolic analysis of Epaltes mexicana by LC-QTOF-MS: Terpenes with activity against human cancer cell lines

IF 2.5 3区 医学 Q3 CHEMISTRY, MEDICINAL
Tamara Juárez-Velázquez , José Arnold González-Garrido , Irma Sánchez-Lombardo , Nelly del Carmen Jiménez-Pérez , Ivonne María Olivares-Corichi , José Rubén García-Sánchez , Oswaldo Hernández-Abreu
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Abstract

Epaltes mexicana is a plant widely used in traditional medicine and as a food in Mexico; however, its phytochemical and pharmacological studies are limited. This study aimed to identify the active secondary metabolites of Epaltes mexicana and determine its cytotoxic activity on cancer cell lines. Three organic extracts were obtained by maceration using n-hexane, dichloromethane, and methanol. The n-hexane extract was fractioned by simple column chromatography. Eight terpenes were annotated in collection 6 (C6) by LC-QTOF-MS using a gradient elution and Electrospray Ionization (ESI) in positive ion mode: 1) Gibberellin A15, 2) farfugin A, 3) dehydromyodesmone, 4) eremopetasitenin A1, 5) hydroxyisonobilin, 6) anhydrocinnzeylanine, 7) nigakilactone H and 8) taxodione. On the other hand, C6 showed a concentration-dependent cytotoxic effect on cancer cell lines MCF-7 (Emax = 74.69 ± 6.19 % and IC50 = 6.31 μg/mL), MDA-MB-231 (Emax = 79.28 ± 12.12 % and IC50 = 124.21 μg/mL), and SiHa (Emax = 82.96 ± 6.02 % and IC50 = 124.31 μg/mL). The C6 did not show a cytotoxic effect against DU-145 and non-cancerous cells from the mammary glands MCF-10A. These results indicate cytotoxic specificity on cancer cell lines and support the hypothesis that terpenes identified in E. mexicana must be investigated and developed for non-clinical and clinical trials as potential anti-cancer drugs.

Abstract Image

利用 LC-QTOF-MS 对 Epaltes mexicana 进行非靶向代谢分析:对人类癌细胞株具有活性的萜烯类化合物。
在墨西哥,Epaltes mexicana 是一种广泛用于传统医药和食品的植物;然而,对它的植物化学和药理学研究却很有限。本研究旨在鉴定墨西哥桉树的活性次生代谢物,并确定其对癌细胞株的细胞毒性活性。研究人员使用正己烷、二氯甲烷和甲醇浸泡,获得了三种有机提取物。正己烷提取物通过简单的柱层析法进行分馏。采用梯度洗脱和正离子模式下的电喷雾离子化(ESI),通过 LC-QTOF-MS 对第 6 组(C6)中的 8 种萜烯类化合物进行了注释:1) 赤霉素 A15,2) 远志霉素 A,3) 脱水毛地黄酮,4) 赤霉素 A1,5) 羟基异异黄酮素,6) 羟基嗪基苯胺,7) 黑内酯 H 和 8) 紫杉二酮。另一方面,C6 对癌细胞株 MCF-7(Emax = 74.69 ± 6.19 %,IC50 = 6.31 μg/mL)、MDA-MB-231(Emax = 79.28 ± 12.12 %,IC50 = 124.21 μg/mL)和 SiHa(Emax = 82.96 ± 6.02 %,IC50 = 124.31 μg/mL)具有浓度依赖性细胞毒性作用。C6 对 DU-145 和来自乳腺 MCF-10 A 的非癌细胞没有细胞毒性作用。这些结果表明了对癌细胞株的细胞毒性特异性,并支持这样的假设,即必须研究和开发在 E. mexicana 中发现的萜类化合物,将其作为潜在的抗癌药物进行非临床和临床试验。
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来源期刊
Fitoterapia
Fitoterapia 医学-药学
CiteScore
5.80
自引率
2.90%
发文量
198
审稿时长
1.5 months
期刊介绍: Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas: 1. Characterization of active ingredients of medicinal plants 2. Development of standardization method for bioactive plant extracts and natural products 3. Identification of bioactivity in plant extracts 4. Identification of targets and mechanism of activity of plant extracts 5. Production and genomic characterization of medicinal plants biomass 6. Chemistry and biochemistry of bioactive natural products of plant origin 7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.
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