Herpesviruses and human papillomaviruses in saliva and biopsies of patients with orofacial tumors.

IF 2.2 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

Clinics Pub Date : 2024-08-31 eCollection Date: 2024-01-01 DOI:10.1016/j.clinsp.2024.100477

Paa-Kwesi Blankson, Grace E Parkins, Harriet Naa Afia Blankson, Abiodun Olubayo Fasola, Prince J Pappoe-Ashong, Matthew O Boamah, Richard Harry Asmah

{"title":"Herpesviruses and human papillomaviruses in saliva and biopsies of patients with orofacial tumors.","authors":"Paa-Kwesi Blankson, Grace E Parkins, Harriet Naa Afia Blankson, Abiodun Olubayo Fasola, Prince J Pappoe-Ashong, Matthew O Boamah, Richard Harry Asmah","doi":"10.1016/j.clinsp.2024.100477","DOIUrl":null,"url":null,"abstract":"Objectives: To determine the prevalence and association of HPV and Herpesviruses in saliva and tissue samples of patients with orofacial tumors.Methods: Biopsies of tumors were done, and saliva samples were collected from patients with orofacial tumors for the determination of viruses using nested multiplex PCR. Independent variables were sex, age, comorbidities, tumor stage, and length of stay. Outcome variables were the presence or absence of herpesviruses and HPV. Descriptive summaries and inferential statistics were done.Results: A hundred patients were included in the study. Prevalence of herpesviruses and HPV were 17.6 % and 57.0 % in tumors, and 48.3 % and 60.0 % in the saliva of patients respectively. Herpesviruses detected included EBV (21.3 %), HHV-7 (11.2 %), CMV (6.7 %), HSV-1 (5.1 %), HSV-2 (1.1 %), VZV (1.1 %), and Kaposi sarcoma virus (0.6 %). The most prevalent HPV genotypes were HPV-42 (29 %), HPV-43 (22.7 %), HPV-52 (22.2 %), HPV-39 (18.8 %), and HPV-18 (9.1 %). The odds of EBV being detected in malignant orofacial tumors were 2 times that of benign orofacial tumors. HPV DNA in the saliva of patients with orofacial tumors was 69.7 %, compared to 18.2 % of the control sample (p < 0.001). The median length of stay for all participants was 6.5 days, those associated with viruses stayed longer.Conclusion: There was a high prevalence of Herpesviruses and HPV in saliva and tumor samples of patients with orofacial tumors, signalling some potential for more work to be done in this area.","PeriodicalId":10472,"journal":{"name":"Clinics","volume":"79 ","pages":"100477"},"PeriodicalIF":2.2000,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11402418/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.clinsp.2024.100477","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives: To determine the prevalence and association of HPV and Herpesviruses in saliva and tissue samples of patients with orofacial tumors.

Methods: Biopsies of tumors were done, and saliva samples were collected from patients with orofacial tumors for the determination of viruses using nested multiplex PCR. Independent variables were sex, age, comorbidities, tumor stage, and length of stay. Outcome variables were the presence or absence of herpesviruses and HPV. Descriptive summaries and inferential statistics were done.

Results: A hundred patients were included in the study. Prevalence of herpesviruses and HPV were 17.6 % and 57.0 % in tumors, and 48.3 % and 60.0 % in the saliva of patients respectively. Herpesviruses detected included EBV (21.3 %), HHV-7 (11.2 %), CMV (6.7 %), HSV-1 (5.1 %), HSV-2 (1.1 %), VZV (1.1 %), and Kaposi sarcoma virus (0.6 %). The most prevalent HPV genotypes were HPV-42 (29 %), HPV-43 (22.7 %), HPV-52 (22.2 %), HPV-39 (18.8 %), and HPV-18 (9.1 %). The odds of EBV being detected in malignant orofacial tumors were 2 times that of benign orofacial tumors. HPV DNA in the saliva of patients with orofacial tumors was 69.7 %, compared to 18.2 % of the control sample (p < 0.001). The median length of stay for all participants was 6.5 days, those associated with viruses stayed longer.

Conclusion: There was a high prevalence of Herpesviruses and HPV in saliva and tumor samples of patients with orofacial tumors, signalling some potential for more work to be done in this area.

查看原文本刊更多论文

口面部肿瘤患者唾液和活检组织中的疱疹病毒和人类乳头瘤病毒。

目的：确定口面部肿瘤患者唾液和组织样本中 HPV 和疱疹病毒的流行率及其关联性：确定口面部肿瘤患者唾液和组织样本中 HPV 和疱疹病毒的流行率和关联性：方法：对肿瘤进行活组织切片检查，并收集口面部肿瘤患者的唾液样本，使用巢式多重 PCR 检测病毒。自变量为性别、年龄、合并症、肿瘤分期和住院时间。结果变量为是否存在疱疹病毒和人乳头瘤病毒。研究进行了描述性总结和推论性统计：研究共纳入 100 名患者。疱疹病毒和人乳头瘤病毒在肿瘤中的感染率分别为 17.6% 和 57.0%，在患者唾液中的感染率分别为 48.3% 和 60.0%。检测到的疱疹病毒包括 EBV（21.3%）、HHV-7（11.2%）、CMV（6.7%）、HSV-1（5.1%）、HSV-2（1.1%）、VZV（1.1%）和卡波西肉瘤病毒（0.6%）。最常见的 HPV 基因型为 HPV-42（29%）、HPV-43（22.7%）、HPV-52（22.2%）、HPV-39（18.8%）和 HPV-18（9.1%）。在恶性口腔肿瘤中检测到 EBV 的几率是良性口腔肿瘤的 2 倍。口面部肿瘤患者唾液中的HPV DNA含量为69.7%，而对照样本中的HPV DNA含量为18.2%（P < 0.001）。所有参与者的住院时间中位数为 6.5 天，与病毒有关的患者住院时间更长：口面部肿瘤患者的唾液和肿瘤样本中疱疹病毒和人乳头瘤病毒的流行率很高，这表明在这一领域有可能开展更多的工作。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Clinics 医学-医学：内科

CiteScore

4.10

自引率

3.70%

发文量

129

审稿时长

52 days

期刊介绍： CLINICS is an electronic journal that publishes peer-reviewed articles in continuous flow, of interest to clinicians and researchers in the medical sciences. CLINICS complies with the policies of funding agencies which request or require deposition of the published articles that they fund into publicly available databases. CLINICS supports the position of the International Committee of Medical Journal Editors (ICMJE) on trial registration.