{"title":"Challenges and possibilities for the qualitative and quantitative analysis of phosphate metabolites","authors":"","doi":"10.1016/j.trac.2024.117934","DOIUrl":null,"url":null,"abstract":"<div><p>Phosphate metabolites (PMs) are endogenous substances that contain phosphate groups, constituting over half of the small molecules in the body. They play crucial roles in various biological processes. However, the qualitative and quantitative analysis of PMs in biological matrices remains challenging. To elucidate the current status of detection methodologies, this review summarizes existing studies on the extraction and analysis of PMs. Emphasis is placed on the chemical derivatization-based mass spectrometry (CD-MS) strategy, with a detailed examination of the types of CD reagents and the reactive groups in PMs. Additionally, emerging methods potentially applicable to PM analysis are reviewed. Finally, we discuss the challenges and future perspectives in this field. This comprehensive review aims to enhance the understanding of PM analysis, thereby facilitating their exploration in health and disease contexts.</p></div>","PeriodicalId":439,"journal":{"name":"Trends in Analytical Chemistry","volume":null,"pages":null},"PeriodicalIF":11.8000,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Trends in Analytical Chemistry","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0165993624004175","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Phosphate metabolites (PMs) are endogenous substances that contain phosphate groups, constituting over half of the small molecules in the body. They play crucial roles in various biological processes. However, the qualitative and quantitative analysis of PMs in biological matrices remains challenging. To elucidate the current status of detection methodologies, this review summarizes existing studies on the extraction and analysis of PMs. Emphasis is placed on the chemical derivatization-based mass spectrometry (CD-MS) strategy, with a detailed examination of the types of CD reagents and the reactive groups in PMs. Additionally, emerging methods potentially applicable to PM analysis are reviewed. Finally, we discuss the challenges and future perspectives in this field. This comprehensive review aims to enhance the understanding of PM analysis, thereby facilitating their exploration in health and disease contexts.
磷酸盐代谢物（PMs）是含有磷酸盐基团的内源性物质，占体内小分子物质的一半以上。它们在各种生物过程中发挥着至关重要的作用。然而，生物基质中磷酸盐代谢物的定性和定量分析仍然具有挑战性。为了阐明检测方法的现状，本综述总结了现有的可吸入颗粒物提取和分析研究。重点介绍了基于化学衍生的质谱分析（CD-MS）策略，并详细分析了 CD 试剂的类型和可吸入颗粒物中的反应基团。此外，还回顾了可能适用于 PM 分析的新兴方法。最后，我们讨论了该领域的挑战和未来展望。这篇全面的综述旨在加深人们对可吸入颗粒物分析的理解，从而促进在健康和疾病背景下对其进行探索。
期刊介绍：
TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.
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