A Miniature Modular Fluorescence Flow Cytometry System.

IF 4.9 3区工程技术 Q1 CHEMISTRY, ANALYTICAL

Biosensors-Basel Pub Date : 2024-08-16 DOI:10.3390/bios14080395

Shaoqi Huang, Jiale Li, Li Wei, Lulu Zheng, Zheng Shi, Shiwei Guo, Bo Dai, Dawei Zhang, Songlin Zhuang

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Abstract

Fluorescence flow cytometry is a powerful instrument to distinguish cells or particles labelled with high-specificity fluorophores. However, traditional flow cytometry is complex, bulky, and inconvenient for users to adjust fluorescence channels. In this paper, we present a modular fluorescence flow cytometry (M-FCM) system in which fluorescence channels can be flexibly arranged. Modules for particle focusing and fluorescence detection were developed. After hydrodynamical focusing, the cells were measured in the detection modules, which were integrated with in situ illumination and fluorescence detection. The signal-to-noise ratio of the detection reached to 33.2 dB. The crosstalk among the fluorescence channels was eliminated. The M-FCM system was applied to evaluate cell viability in drug screening, agreeing well with the commercial cytometry. The modular cytometry presents several outstanding features: flexibility in setting fluorescence channels, cost efficiency, compact construction, ease of operation, and the potential to upgrade for multifunctional measurements. The modular cytometry provides a multifunctional platform for various biophysical measurements, e.g., electrical impedance and refractive-index detection. The proposed work paves an innovative avenue for the multivariate analysis of cellular characteristics.

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微型模块化荧光流式细胞仪系统

荧光流式细胞仪是一种功能强大的仪器，可用于区分被高特异性荧光团标记的细胞或颗粒。然而，传统的流式细胞仪结构复杂、体积庞大，而且用户不方便调整荧光通道。在本文中，我们介绍了一种模块化荧光流式细胞仪（M-FCM）系统，其中的荧光通道可以灵活排列。我们开发了用于粒子聚焦和荧光检测的模块。流体力学聚焦后，细胞在检测模块中进行测量，检测模块集成了原位照明和荧光检测功能。检测的信噪比达到 33.2 dB。消除了荧光通道之间的串扰。M-FCM 系统被应用于评估药物筛选中的细胞活力，与商业化的细胞测量法不谋而合。模块化细胞仪具有几个突出的特点：灵活设置荧光通道、成本效益高、结构紧凑、操作简便，以及可升级进行多功能测量。模块化细胞仪为电阻抗和折射率检测等各种生物物理测量提供了多功能平台。拟议的工作为细胞特性的多元分析铺平了一条创新之路。

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来源期刊

Biosensors-Basel Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

CiteScore

6.60

自引率

14.80%

发文量

983

审稿时长

11 weeks

期刊介绍： Biosensors (ISSN 2079-6374) provides an advanced forum for studies related to the science and technology of biosensors and biosensing. It publishes original research papers, comprehensive reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.