Vitamin C Improves Oocyte In Vitro Maturation and Potentially Changes Embryo Quality in Cattle.

IF 2 2区农林科学 Q2 VETERINARY SCIENCES

Veterinary Sciences Pub Date : 2024-08-13 DOI:10.3390/vetsci11080372

Yueqi Wang, Aibing Wang, Hongmei Liu, Rui Yang, Boyang Zhang, Bo Tang, Ziyi Li, Xueming Zhang

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引用次数: 0

Abstract

To obtain high-quality bovine oocytes, the effects of vitamin C (VC) on the IVM of bovine oocytes and early embryo development were investigated. The results showed the following. (1) The IVM medium containing 50 µg/mL VC improved the oocyte maturation rate but did not affect the parthenogenetic embryo development. (2) The IVC medium containing 20 µg/mL VC improved the cleavage rate of the IVF embryos and enhanced the mRNA transcriptions of pluripotency gene Oct4, Sox2, Cdx2, and Nanog in the blastocysts but had no effects on the blastocyst rate. (3) Combining supplementation of 50 µg/mL VC in IVM medium + 20 µg/mL VC in IVC medium (named as VC 50/20, similar hereinafter) elevated the cleavage rate of IVF embryos and enhanced the mRNA expressions of Oct4, Sox2, Cdx2, and Nanog in the blastocysts. (4) Combination of VC 0/20 and VC 50/20 enhanced the transcription of anti-apoptotic gene Bcl-2 and VC 50/0 weakened the transcription of pro-apoptotic gene Bax, while VC 0/40 and VC 0/60 increased Bax expression and diminished the Bcl-2/Bax ratio in blastocysts. Together, employing 50 µg/mL VC improves the IVM of bovine oocytes and combination of VC 50/20 potentially changes bovine embryo quality by enhancing the expressions of the pluripotency genes and regulating the expressions of apoptosis-related genes.

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维生素 C 可改善牛卵母细胞的体外成熟并潜在地改变胚胎质量。

为了获得高质量的牛卵母细胞，研究了维生素 C（VC）对牛卵母细胞 IVM 和早期胚胎发育的影响。结果如下(1）含 50 µg/mL VC 的 IVM 培养基可提高卵母细胞成熟率，但不影响孤雌胚发育。(2）含 20 µg/mL VC 的 IVC 培养基提高了试管婴儿胚胎的裂殖率，并增强了囊胚中多能基因 Oct4、Sox2、Cdx2 和 Nanog 的 mRNA 转录，但对囊胚率没有影响。(3）在 IVM 培养基中添加 50 µg/mL VC + 在 IVC 培养基中添加 20 µg/mL VC（命名为 VC 50/20，下同）可提高 IVF 胚胎的裂解率，并增强囊胚中 Oct4、Sox2、Cdx2 和 Nanog 的 mRNA 表达。(4) 结合使用 VC 0/20 和 VC 50/20 可增强抗凋亡基因 Bcl-2 的转录，VC 50/0 可削弱促凋亡基因 Bax 的转录，而 VC 0/40 和 VC 0/60 可增加 Bax 的表达并降低囊胚中的 Bcl-2/Bax 比率。总之，使用 50 µg/mL 的 VC 可改善牛卵母细胞的 IVM，而 VC 50/20 的组合可通过增强多能基因的表达和调节凋亡相关基因的表达来改变牛胚胎的质量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Veterinary Sciences VETERINARY SCIENCES-

CiteScore

2.90

自引率

8.30%

发文量

612

审稿时长

6 weeks

期刊介绍： Veterinary Sciences is an international and interdisciplinary scholarly open access journal. It publishes original that are relevant to any field of veterinary sciences, including prevention, diagnosis and treatment of disease, disorder and injury in animals. This journal covers almost all topics related to animal health and veterinary medicine. Research fields of interest include but are not limited to: anaesthesiology anatomy bacteriology biochemistry cardiology dentistry dermatology embryology endocrinology epidemiology genetics histology immunology microbiology molecular biology mycology neurobiology oncology ophthalmology parasitology pathology pharmacology physiology radiology surgery theriogenology toxicology virology.