[Involvement of microRNA-145/TGF-β in the regulation of swimming exercise on vascular calcification in type 2 diabetic rats].

Q3 Medicine
生理学报 Pub Date : 2024-08-25
Gang-Gang Xu, Chun-Lian Ma, Yi Yang
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引用次数: 0

Abstract

The present study aimed to explore the effect of swimming exercise on vascular calcification in type 2 diabetic rats and its related molecular mechanism. Male Sprague Dawley (SD) rats were randomly divided into normal control (NC), diabetes control (DC) and diabetes+exercise (DE) groups. The DC and DE groups were intraperitoneally injected with streptozotocin (STZ) and fed with high-fat diet to establish type 2 diabetes mellitus model. The NC and DC groups did not exercise, and the DE group performed swimming exercise for 8 weeks. ELISA was used to detect the serum glycated hemoglobin A1c (HbA1c) level. The aortas of rats were taken as sample. Assay kits were used to detect vascular calcium content and alkaline phosphatase (ALP) activity. Von Kossa staining was used to detect calcium deposition. qRT-PCR was used detect the expression of microRNA-145 (miR-145). Western blot was used to detect the protein expression levels of smooth muscle contraction markers, calcification marker and related proteins. The results showed that, compared with the NC group, the blood glucose, serum HbA1c level, vascular calcium content and ALP activity in the DC group were significantly increased, the protein expression levels of smooth muscle contraction markers smooth muscle protein 22α (SM22α) and α-smooth muscle actin (α-SMA) were significantly down-regulated, and the protein expression level of calcification marker osteopontin (OPN) was significantly up-regulated; Compared with the DC group, the serum HbA1c level, vascular calcium content and ALP activity in the DE group were significantly decreased, the protein expression levels of SM22α and α-SMA were significantly up-regulated, and the protein expression level of OPN was significantly down-regulated; Compared with the NC group, the expression of miR-145-5p in the DC group was significantly down-regulated, and the protein expression levels of transforming growth factor-β (TGF-β), SMAD2, ERK1/2 and p-ERK1/2 were significantly up-regulated; Compared with the DC group, the expression of miR-145-5p was significantly up-regulated in the DE group, while the expressions of TGF-β, ERK1/2 and p-ERK1/2 were significantly down-regulated. These results suggest that miR-145/TGF-β signaling is involved in the improving effects of 8-week swimming exercise on glucose metabolism disorder, vascular smooth muscle cell phenotype switching and vascular calcification in type 2 diabetes mellitus.

[microRNA-145/TGF-β参与调节游泳运动对2型糖尿病大鼠血管钙化的影响]
本研究旨在探讨游泳运动对2型糖尿病大鼠血管钙化的影响及其相关分子机制。将雄性Sprague Dawley(SD)大鼠随机分为正常对照组(NC)、糖尿病对照组(DC)和糖尿病+运动组(DE)。DC组和DE组大鼠腹腔注射链脲佐菌素(STZ)并以高脂饮食喂养,以建立2型糖尿病模型。NC组和DC组不运动,DE组进行为期8周的游泳运动。用 ELISA 检测血清糖化血红蛋白 A1c(HbA1c)水平。取大鼠主动脉作为样本。检测试剂盒用于检测血管钙含量和碱性磷酸酶(ALP)活性。qRT-PCR 用于检测 microRNA-145 (miR-145) 的表达。Western blot 用于检测平滑肌收缩标志物、钙化标志物及相关蛋白的表达水平。结果显示,与NC组相比,DC组的血糖、血清HbA1c水平、血管钙含量和ALP活性显著升高,平滑肌收缩标志物平滑肌蛋白22α(SM22α)和α-平滑肌肌动蛋白(α-SMA)的蛋白表达水平显著下调,而钙化标志物骨化蛋白(OPN)的蛋白表达水平显著上调;与 DC 组相比,DE 组血清 HbA1c 水平、血管钙含量和 ALP 活性明显降低,SM22α 和 α-SMA 蛋白表达水平明显上调,OPN 蛋白表达水平明显下调;与 NC 组相比,DC 组 miR-145-5p 的表达明显下调,而转化生长因子-β(TGF-β)、SMAD2、ERK1/2 和 p-ERK1/2 的蛋白表达水平明显上调;与 DC 组相比,DE 组 miR-145-5p 的表达明显上调,而 TGF-β、ERK1/2 和 p-ERK1/2 的表达明显下调。这些结果表明,miR-145/TGF-β信号转导参与了8周游泳运动对2型糖尿病患者糖代谢紊乱、血管平滑肌细胞表型转换和血管钙化的改善作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
生理学报
生理学报 Medicine-Medicine (all)
CiteScore
1.20
自引率
0.00%
发文量
4820
期刊介绍: Acta Physiologica Sinica (APS) is sponsored by the Chinese Association for Physiological Sciences and Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences (CAS), and is published bimonthly by the Science Press, China. APS publishes original research articles in the field of physiology as well as research contributions from other biomedical disciplines and proceedings of conferences and symposia of physiological sciences. Besides “Original Research Articles”, the journal also provides columns as “Brief Review”, “Rapid Communication”, “Experimental Technique”, and “Letter to the Editor”. Articles are published in either Chinese or English according to authors’ submission.
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