{"title":"Is there any relationship between the effectiveness of some Heterorhabditis bacteriophora isolates on the host and their average penetration rate?","authors":"","doi":"10.1016/j.jspr.2024.102401","DOIUrl":null,"url":null,"abstract":"<div><p>Entomopathogenic nematodes, such as <em>Heterorhabditis bacteriophora</em>, have been widely recognized for their potential in biological control strategies to manage agricultural pests effectively. This study aimed to evaluate the penetration rate and efficacy of different <em>H. bacteriophora</em> isolates, including two isolates (HB4 and HBNL) and a hybrid strain (HBH), against <em>Tenebrio molitor</em> L. (Col: Tenebrionidae) adults. The research also aimed to determine whether there was a correlation between the mortality caused by these isolates and their penetration. These isolates were applied to <em>T. molitor</em> adults at different concentrations 0.78 IJs (infective juveniles)/cm<sup>2</sup> (50 IJs/Petri dishes), 1.57 IJs/cm<sup>2</sup> (100 IJs/Petri dishes), 2.36 IJs/cm<sup>2</sup> (150 IJs/Petri dishes), 3.14 IJs/cm<sup>2</sup> (200 IJs/Petri dishes), 3.93 IJs/cm<sup>2</sup> (250 IJs/Petri dishes), 7.86 IJs/cm<sup>2</sup> (500 IJs/Petri dishes), 11.8 IJs/cm<sup>2</sup> (750 IJs/Petri dishes), 15.72 IJs/cm<sup>2</sup> (1000 IJs/Petri dishes), 19.66 IJs/cm<sup>2</sup> (1250 IJs/Petri dishes). Another objective of the study was to observe the penetration rate means of these nematodes at consistent concentrations and record the resulting mortality rates. The findings revealed that the highest mortality rate of 100% was achieved at concentrations of 250, 500, 750, 1000, and 1250 IJs/Petri dishes. The HBH hybrid strain also exhibited the highest penetration rate mean of 20.33 IJs at a concentration of 1250 IJs/Petri dishes. These results highlight the efficacy of <em>H. bacteriophora</em> isolates and the hybrid strain in controlling <em>T. molitor</em> adults, with significant mortality rates observed at higher nematode concentrations. The study's emphasis on penetration rate means and the relationship between nematode concentration and pest mortality underscores the importance of optimizing entomopathogenic nematode applications for effective pest management.</p></div>","PeriodicalId":17019,"journal":{"name":"Journal of Stored Products Research","volume":null,"pages":null},"PeriodicalIF":2.7000,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Stored Products Research","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0022474X24001589","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Entomopathogenic nematodes, such as Heterorhabditis bacteriophora, have been widely recognized for their potential in biological control strategies to manage agricultural pests effectively. This study aimed to evaluate the penetration rate and efficacy of different H. bacteriophora isolates, including two isolates (HB4 and HBNL) and a hybrid strain (HBH), against Tenebrio molitor L. (Col: Tenebrionidae) adults. The research also aimed to determine whether there was a correlation between the mortality caused by these isolates and their penetration. These isolates were applied to T. molitor adults at different concentrations 0.78 IJs (infective juveniles)/cm2 (50 IJs/Petri dishes), 1.57 IJs/cm2 (100 IJs/Petri dishes), 2.36 IJs/cm2 (150 IJs/Petri dishes), 3.14 IJs/cm2 (200 IJs/Petri dishes), 3.93 IJs/cm2 (250 IJs/Petri dishes), 7.86 IJs/cm2 (500 IJs/Petri dishes), 11.8 IJs/cm2 (750 IJs/Petri dishes), 15.72 IJs/cm2 (1000 IJs/Petri dishes), 19.66 IJs/cm2 (1250 IJs/Petri dishes). Another objective of the study was to observe the penetration rate means of these nematodes at consistent concentrations and record the resulting mortality rates. The findings revealed that the highest mortality rate of 100% was achieved at concentrations of 250, 500, 750, 1000, and 1250 IJs/Petri dishes. The HBH hybrid strain also exhibited the highest penetration rate mean of 20.33 IJs at a concentration of 1250 IJs/Petri dishes. These results highlight the efficacy of H. bacteriophora isolates and the hybrid strain in controlling T. molitor adults, with significant mortality rates observed at higher nematode concentrations. The study's emphasis on penetration rate means and the relationship between nematode concentration and pest mortality underscores the importance of optimizing entomopathogenic nematode applications for effective pest management.
期刊介绍:
The Journal of Stored Products Research provides an international medium for the publication of both reviews and original results from laboratory and field studies on the preservation and safety of stored products, notably food stocks, covering storage-related problems from the producer through the supply chain to the consumer. Stored products are characterised by having relatively low moisture content and include raw and semi-processed foods, animal feedstuffs, and a range of other durable items, including materials such as clothing or museum artefacts.