Detection of Leishmania RNA Virus 2 (LRV2) among Clinical Isolates of Leishmania Major in Four Endemic Regions of Iran

IF 1.2 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2024-08-27 DOI:10.1007/s11686-024-00915-x

Samaneh Mazaherifar, Saiedeh Erfanian, Kavous Solhjoo, Abazar Roustazadeh, Mohammad Darayesh, Ali Taghipour, Shahab Falahi, Azra Kenarkoohi, Milad Badri, Seyede Manizhe Heidarnejadi, Sima Rasti, Amir Abdoli

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Abstract

Purpose

Leishmania RNA viruses (LRV) are double-stranded RNA viruses (dsRNA viruses) that play a role in the pathogenesis of Leishmania parasites. Cutaneous leishmaniasis (CL) is endemic in various parts of Iran. Our aimed was to investigate presence of LRV among the Leishmania major isolates in four endemic regions of Iran.

Methods

In a cross-sectional study, we assessed the presence of LRV1 and LRV2 in 181 clinical isolates of L. major from four endemic cities in Iran using reverse transcription polymerase chain reaction (RT-PCR). After RNA extraction and cDNA synthesis, RT-PCR tests were conducted with LRV1 and LRV2 specific primers. Human beta-actin and kmp genes served as internal and external controls, respectively, and the Allele ID software was used to optimize melting curves.

Results

LRV2 was detected in 27.6% (50 out of 181) of L. major isolates, while no LRV1 was found. We did not observe a statistically significant difference in the presence of LRV2 based on age group, number, or location of lesions.

Conclusion

This study confirms the presence of LRV2 in clinical isolates of L. major from endemic regions of Iran. Further researches with larger sample sizes is recommended to explore the association between LRV and clinical symptoms as well as treatment response.

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在伊朗四个利什曼病流行地区的大利什曼病临床分离株中检测利什曼病 RNA 病毒 2 (LRV2)。

目的：利什曼病 RNA 病毒（LRV）是一种双链 RNA 病毒（dsRNA 病毒），在利什曼病寄生虫的致病过程中发挥作用。皮肤利什曼病（CL）在伊朗各地流行。我们的目的是调查伊朗四个流行地区利什曼病主要分离株中是否存在 LRV：在一项横断面研究中，我们使用反转录聚合酶链反应（RT-PCR）评估了来自伊朗四个流行城市的 181 例临床分离大利什曼原虫中 LRV1 和 LRV2 的存在情况。提取 RNA 并合成 cDNA 后，使用 LRV1 和 LRV2 特异引物进行 RT-PCR 检测。人类 beta-肌动蛋白和 kmp 基因分别作为内部和外部对照，并使用 Allele ID 软件优化熔解曲线：结果：27.6%的大鼠分离株（181 株中有 50 株）检测到 LRV2，而没有发现 LRV1。我们没有观察到不同年龄组、病变数量或位置的 LRV2 存在显著的统计学差异：本研究证实，在伊朗地方病流行地区的大鼠临床分离物中存在 LRV2。建议进一步开展样本量更大的研究，探讨 LRV 与临床症状及治疗反应之间的关联。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.