Single-Cell Sequencing: High-Resolution Analysis of Cellular Heterogeneity in Autoimmune Diseases.

IF 8.4 2区 医学 Q1 ALLERGY
Clinical Reviews in Allergy & Immunology Pub Date : 2024-06-01 Epub Date: 2024-08-26 DOI:10.1007/s12016-024-09001-6
Xuening Tang, Yudi Zhang, Hao Zhang, Nan Zhang, Ziyu Dai, Quan Cheng, Yongzhen Li
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Abstract

Autoimmune diseases (AIDs) are complex in etiology and diverse in classification but clinically show similar symptoms such as joint pain and skin problems. As a result, the diagnosis is challenging, and usually, only broad treatments can be available. Consequently, the clinical responses in patients with different types of AIDs are unsatisfactory. Therefore, it is necessary to conduct more research to figure out the pathogenesis and therapeutic targets of AIDs. This requires research technologies with strong extraction and prediction capabilities. Single-cell sequencing technology analyses the genomic, epigenomic, or transcriptomic information at the single-cell level. It can define different cell types and states in greater detail, further revealing the molecular mechanisms that drive disease progression. These advantages enable cell biology research to achieve an unprecedented resolution and scale, bringing a whole new vision to life science research. In recent years, single-cell technology especially single-cell RNA sequencing (scRNA-seq) has been widely used in various disease research. In this paper, we present the innovations and applications of single-cell sequencing in the medical field and focus on the application contributing to the differential diagnosis and precise treatment of AIDs. Despite some limitations, single-cell sequencing has a wide range of applications in AIDs. We finally present a prospect for the development of single-cell sequencing. These ideas may provide some inspiration for subsequent research.

Abstract Image

单细胞测序:高分辨率分析自身免疫性疾病的细胞异质性。
自身免疫性疾病(AIDs)的病因复杂,分类多样,但临床症状相似,如关节疼痛和皮肤问题。因此,诊断具有挑战性,通常只能提供广泛的治疗方法。因此,不同类型艾滋病患者的临床反应并不令人满意。因此,有必要开展更多的研究,找出艾滋病的发病机制和治疗目标。这就需要具有强大提取和预测能力的研究技术。单细胞测序技术在单细胞水平上分析基因组、表观基因组或转录组信息。它可以更详细地定义不同的细胞类型和状态,进一步揭示驱动疾病进展的分子机制。这些优势使细胞生物学研究达到了前所未有的分辨率和规模,为生命科学研究带来了全新的视野。近年来,单细胞技术尤其是单细胞 RNA 测序(scRNA-seq)已广泛应用于各种疾病研究。本文将介绍单细胞测序技术在医学领域的创新和应用,并重点介绍其在艾滋病鉴别诊断和精准治疗方面的应用。尽管存在一些局限性,单细胞测序在艾滋病领域仍有广泛的应用前景。最后,我们展望了单细胞测序的发展前景。这些观点或许能为后续研究提供一些启发。
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来源期刊
CiteScore
22.30
自引率
1.10%
发文量
58
审稿时长
6-12 weeks
期刊介绍: Clinical Reviews in Allergy & Immunology is a scholarly journal that focuses on the advancement of clinical management in allergic and immunologic diseases. The journal publishes both scholarly reviews and experimental papers that address the current state of managing these diseases, placing new data into perspective. Each issue of the journal is dedicated to a specific theme of critical importance to allergists and immunologists, aiming to provide a comprehensive understanding of the subject matter for a wide readership. The journal is particularly helpful in explaining how novel data impacts clinical management, along with advancements such as standardized protocols for allergy skin testing and challenge procedures, as well as improved understanding of cell biology. Ultimately, the journal aims to contribute to the improvement of care and management for patients with immune-mediated diseases.
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