Enhancing seed oil content and fatty acid composition in camelina through overexpression of castor RcWRI1A and RcMYB306

IF 2.3 3区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Applied Biological Chemistry Pub Date : 2024-08-24 DOI:10.1186/s13765-024-00927-1

Mid-Eum Park, Inyoung Kim, Hye Ji Lee, Mi Chung Suh, Kyeong-Ryeol Lee, Hyun Uk Kim

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引用次数: 0

Abstract

Seed triacylglycerol (TAG), a major component of vegetable oil, consists of a glycerol esterified with three fatty acids. Vegetable oil has industrial applications and is widely used as edible oil. The increasing demand for plant oils, owing to population growth, it is crucial to enhance the oil content in seeds. We found castor WRINKLED1A (RcWRI1A) and R2R3-type MYB domain protein 306 (RcMYB306) which have homology with Arabidopsis WRI1 (AtWRI1) and AtMYB96 which regulate genes involved in fatty acid and TAG synthesis, respectively. These castor genes were separately and jointly overexpressed using seed-specific promoters in an oil crop, camelina (Camelina sativa). Overexpression of RcWRI1A, RcMYB306, or RcWRI1A + RcMYB306 increased the total seed oil content in camelina. However, this increase was not significantly different from that observed during the overexpression of RcWRI1A or/and RcMYB306. RcWRI1A overexpression increased the fatty acid content, including 16:0, 18:2, 18:3. Contrastingly, RcMYB306 overexpression increased the 18:1, 18:2, 18:3, 20:0 and 20:1 fatty acid. In the RcWRI1A + RcMYB306 lines, changes in fatty acid composition demonstrated the combined effects of these transcription factors. These results suggest that RcWRI1A and RcMYB306 can be used to improve the productivity of oil crops.

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通过过表达蓖麻 RcWRI1A 和 RcMYB306 提高荠菜籽油含量和脂肪酸组成

种子三酰甘油（TAG）是植物油的主要成分，由甘油与三种脂肪酸酯化而成。植物油具有工业用途，被广泛用作食用油。由于人口增长，对植物油的需求不断增加，因此提高种子中的含油量至关重要。我们发现蓖麻 WRINKLED1A（RcWRI1A）和 R2R3 型 MYB 结构域蛋白 306（RcMYB306）与拟南芥 WRI1（AtWRI1）和 AtMYB96 具有同源性，它们分别调控参与脂肪酸和 TAG 合成的基因。在油料作物荠菜（Camelina sativa）中使用种子特异性启动子分别和联合过表达了这些蓖麻基因。过表达 RcWRI1A、RcMYB306 或 RcWRI1A + RcMYB306 增加了荠菜种子的总含油量。然而，这种增加与过表达 RcWRI1A 或/和 RcMYB306 时观察到的增加没有明显差异。RcWRI1A 的过表达增加了脂肪酸含量，包括 16:0、18:2 和 18:3。相反，RcMYB306 的过表达增加了 18:1、18:2、18:3、20:0 和 20:1 脂肪酸的含量。在 RcWRI1A + RcMYB306 株系中，脂肪酸组成的变化显示了这些转录因子的共同作用。这些结果表明，RcWRI1A 和 RcMYB306 可用于提高油料作物的产量。

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来源期刊

Applied Biological Chemistry Chemistry-Organic Chemistry

CiteScore

5.40

自引率

6.20%

发文量

审稿时长

20 weeks

期刊介绍： Applied Biological Chemistry aims to promote the interchange and dissemination of scientific data among researchers in the field of agricultural and biological chemistry. The journal covers biochemistry and molecular biology, medical and biomaterial science, food science, and environmental science as applied to multidisciplinary agriculture.