Different patterns of fine-root exudation rates along soil depth of Pinus densiflora, Chamaecyparis obtusa, and Cryptomeria japonica in coniferous forests

Abstract

Tree fine-root exudation and organic carbon compounds in the soil affect physiological functions through degradation by microorganisms around the roots and the enhancement of allelopathic effects on invasion attempts by other plant species. However, the underlying mechanism of fine-root action in vertically heterogeneous resource-distribution patterns along the soil profile is little known. Here, we quantified root exudation and its morphological and chemical traits in the soil layers down to 100-cm depth for three conifer species, Pinus densiflora (ectomycorrhiza), Chamaecyparis obtusa, and Cryptomeria japonica (arbuscular mycorrhiza), in a cool temperate Japanese forest. By using glass filter-trap method, root exudation rates in all three species varied with soil depth in a species dependent pattern. Specifically, the root exudation rate of P. densiflora was significantly higher in the middle soil layer, and lower in the topsoil layer, whereas the exudation rates significantly decreased in C. japonica and did not vary in C. obtusa along soil depth. Exudation strongly correlated with variation in root traits in C. obtusa and C. japonica but not in P. densiflora. These findings suggest that differences in exudation patterns among tree species are likely compensated for by structural modifications in fine roots in response to soil depth. In addition, the traits with mycorrhizal types might be related to adequate root exudation and enhanced soil-nutrient acquisition. Distinct differences among species in exudation, associated with vertical root distribution, will increase our understanding of root survival strategies for nutrient acquisition and carbon sequestration in forest tree species.