Response of bovine endometrium to interferon tau in the presence of lipopolysaccharide

IF 2.4 2区 农林科学 Q3 REPRODUCTIVE BIOLOGY
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Abstract

We recently demonstrated that conceptus-derived interferon tau (IFNT), responsible for maternal recognition in cattle, acts on the uterus in a dose- and time-dependent manner by upregulating key interferon-stimulated genes (ISGs) in the endometrium. In high producing dairy cows, postpartum uterine infection is a major factor influencing fertility and pregnancy outcome. Lipopolysaccharide (LPS), an endotoxin of Gram-negative bacteria such as Escherichia coli, generates an altered uterine environment by inducing excessive inflammation at the maternal-conceptus interface. Thus, we aimed to investigate whether the endometrial response to IFNT is altered in the presence of LPS. Endometrial explants were isolated from uteri collected at a local abattoir from Holstein Friesian cows (n = 8) during the mid-luteal stage of the estrous cycle, and cultured in RPMI medium for 24 h in 5 % CO2 in humidified air without (control), or with IFNT (100 ng/mL), a single Day 15 conceptus, LPS (1 μg/mL), both IFNT and LPS, or both a Day 15 conceptus and LPS. Incubation with IFNT and a Day 15 conceptus up-regulated (P < 0.05) well-known classical ISGs (ISG15, OAS1, MX1 and MX2) as well as other candidate ISGs (CMPK2, IFI35, TRIM38 and TNFSF10) and down-regulated expression of IL1B in endometrial explants. Incubation with LPS increased (P < 0.05) abundance of NFKB1 (a key transcription factor involved in inflammatory and immune response), TNFA, IL1B and IL6 (pro-inflammatory cytokines), IL10 (anti-inflammatory cytokine), IL8, CXCL1, CXCL3 and CCL2 (chemokines), and, to a lesser extent, classical ISGs in endometrial explants. However, LPS did not alter endometrial response to IFNT, irrespective of IFNT concentration (1, 10 or 100 ng/mL). Results suggest that the expression of ISGs, up-regulated by conceptus-derived IFNT, is not altered in the endometrium in the presence of LPS; however, the increased expression of inflammation-related genes induced by LPS indicate an altered endometrial immune response that may be associated with compromised pregnancy establishment or pregnancy failure.

脂多糖存在时牛子宫内膜对 tau 干扰素的反应
我们最近证实,牛的母体识别是由受体衍生的干扰素 tau(IFNT)引起的,它通过上调子宫内膜中的关键干扰素刺激基因(ISGs),以剂量和时间依赖的方式作用于子宫。在高产奶牛中,产后子宫感染是影响繁殖力和妊娠结果的一个主要因素。脂多糖(LPS)是革兰氏阴性细菌(如大肠杆菌)的一种内毒素,可通过诱导母体-子宫腔界面的过度炎症而改变子宫环境。因此,我们旨在研究在 LPS 存在的情况下,子宫内膜对 IFNT 的反应是否会发生改变。从当地屠宰场收集的发情周期黄体中期荷斯坦弗里斯兰奶牛(n = 8)的子宫中分离出子宫内膜外植体,在RPMI培养基中培养24小时,培养过程中不添加(对照组)或添加IFNT(100纳克/毫升)、第15天的单个受体、LPS(1微克/毫升)、IFNT和LPS或第15天的受体和LPS。与 IFNT 和第 15 天孕卵一起孵育会上调(P < 0.05)众所周知的经典 ISGs(ISG15、OAS1、MX1 和 MX2)以及其他候选 ISGs(CMPK2、IFI35、TRIM38 和 TNFSF10),并下调子宫内膜外植体中 IL1B 的表达。LPS孵育增加了NFKB1(一种参与炎症和免疫反应的关键转录因子)、TNFA、IL1B和IL6(促炎细胞因子)、IL10(抗炎细胞因子)、IL8、CXCL1、CXCL3和CCL2(趋化因子)的丰度(P < 0.05),并在较小程度上增加了子宫内膜外植体中经典ISGs的丰度。然而,无论 IFNT 浓度(1、10 或 100 纳克/毫升)如何,LPS 都不会改变子宫内膜对 IFNT 的反应。研究结果表明,在LPS存在的情况下,ISGs的表达不会改变子宫内膜,而ISGs的表达会被受体衍生的IFNT上调;然而,LPS诱导的炎症相关基因的表达增加表明子宫内膜的免疫反应发生了改变,这可能与妊娠建立受损或妊娠失败有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Theriogenology
Theriogenology 农林科学-生殖生物学
CiteScore
5.50
自引率
14.30%
发文量
387
审稿时长
72 days
期刊介绍: Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.
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