Plant, bacteria and fungi crosstalk: Direct and indirect biocontrol mechanisms of sugarcane rhizoplane Pseudomonas species against Fusarium wilt

Abstract

In order to identify an effective rhizoplane Pseudomonas species to combat sugarcane wilt pathogen (Fusarium sacchari), twenty Pseudomonas isolates were obtained from the sugarcane rhizoplane of different genotypes. The three-tier confrontation (direct, indirect and remote) tests revealed antagonistic activity of the bacteria, bacterial non-volatile and volatile compounds. In dual culture assay, SRP19 (46.30%) and SRP20 (39.30%) have shown the highest inhibition against F. sacchari. 16S-23S rDNA sequencing identified them as Pseudomonas chlororaphis subsp. aurantiaca (SRP19) and Pseudomonas aeruginosa (SRP20). Exploration of biocontrol mechanisms revealed the potential roles for IAA production, extracellular enzyme secretion (proteases, cellulases and chitinases), HCN synthesis, siderophore production, pyocyanin or fluorescein production and phosphate solubilization. The molecular analysis revealed the presence of genes (phz, prnD, phlD and pltC) involved in the biosynthesis of antimicrobial peptides like phenazines, pyrrolnitrin, 2,4-diacetyl phloroglucinol and pyoleuteorin, involved in suppressing Fusarium. The antagonistic activity was further confirmed under greenhouse conditions by sett treatment with potential antagonistic isolates, which increased germination and reduced the pre-emergent mortality by 60% and post-emergent seedling mortality by 76% in SRP19 treated pots. The study revealed that the isolate Pseudomonas chlororaphis subsp. aurantiaca strain SRP19 can act as PGPR with both plant growth promoting and pathogen suppressing activity that can be used as an effective biocontrol agent against Fusarium wilt of sugarcane.