Genetic characterization of white onion genotypes (Allium cepa L.) using ISSR markers

Q3 Agricultural and Biological Sciences

Ecological Genetics and Genomics Pub Date : 2024-08-20 DOI:10.1016/j.egg.2024.100287

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Abstract

Understanding the genetic characteristics is crucial for preserving and developing Moroccan's white onion genotypes, with increased yield and quality as well as improved tolerance to biotic and abiotic stresses. In the present paper, inter simple sequence repeat (ISSR) markers were used to assess the genetic diversity and characteristics of white onion genotypes (Allium cepa L.) of Morocco. Based on the ISSR markers bands, 28 white onion genotypes were divided into four distinguished subgroups, with a genetic similarity value of 0.46 based on Jaccard index. Molecular markers analysis revealed that all ISSR markers were 100 % polymorphic with the white onions genotypes. Concerning markers performance, polymorphic information content (PIC) ranges from 0.051 to 0.39 with a mean of 0.301; however, the effective multiplex ratio (EMR) and marker index (MI) vary between 3–17 and 0.35–5.04, respectively. Based on resolving power (RP), it appears that the marker UBC825 has an efficient discriminatory power with an RP = 15.67. In contrast, the lowest RP value was detected with the marker UBC815 (RP = 1.58). Based on their high performance, markers UBC825, UBC835, UBC826, UBC840, and UBC811 ISSR seem to be specific and efficient for the identification of Moroccan white onion genotypes. Thus, given the genetic diversity shown by ISSR markers, the abundant resources of white onion genotypes in Morocco could be exploited in more valuable ways.

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利用 ISSR 标记鉴定白葱基因型（Allium cepa L.）

了解遗传特征对于保护和发展摩洛哥白葱基因型，提高产量和质量以及改善对生物和非生物胁迫的耐受性至关重要。本文使用简单序列间重复（ISSR）标记来评估摩洛哥白葱基因型（Allium cepa L.）的遗传多样性和特征。根据 ISSR 标记带，28 个白洋葱基因型被分为 4 个不同的亚群，根据 Jaccard 指数，遗传相似度值为 0.46。分子标记分析表明，所有 ISSR 标记与白葱基因型的多态性均为 100%。在标记性能方面，多态信息含量（PIC）介于 0.051 至 0.39 之间，平均值为 0.301；但有效复用比（EMR）和标记指数（MI）分别介于 3-17 和 0.35-5.04 之间。根据分辨力（RP），标记物 UBC825 似乎具有有效的分辨力，RP = 15.67。相比之下，标记物 UBC815 的 RP 值最低（RP = 1.58）。基于 UBC825、UBC835、UBC826、UBC840 和 UBC811 ISSR 标记的高性能，它们在鉴定摩洛哥白葱基因型方面似乎具有特异性和高效性。因此，鉴于 ISSR 标记所显示的遗传多样性，摩洛哥丰富的白葱基因型资源可以得到更有价值的利用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ecological Genetics and Genomics Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： Ecological Genetics and Genomics publishes ecological studies of broad interest that provide significant insight into ecological interactions or/ and species diversification. New data in these areas are published as research papers, or methods and resource reports that provide novel information on technologies or tools that will be of interest to a broad readership. Complete data sets are shared where appropriate. The journal also provides Reviews, and Perspectives articles, which present commentary on the latest advances published both here and elsewhere, placing such progress in its broader biological context. Topics include: -metagenomics -population genetics/genomics -evolutionary ecology -conservation and molecular adaptation -speciation genetics -environmental and marine genomics -ecological simulation -genomic divergence of organisms