Optimization of the determination of volatile organic compounds in plant tissue and soil samples: Untargeted metabolomics of main active compounds

IF 1.6 Q2 MULTIDISCIPLINARY SCIENCES

MethodsX Pub Date : 2024-08-14 DOI:10.1016/j.mex.2024.102914

Nipapan Kanjana , Muhammad Afaq Ahmed , Zhongjian Shen , Yuyan Li , Lisheng Zhang

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Abstract

This review critically assesses the determination of low molecular weight volatiles by different methods, providing context for the development of suitable techniques to determine volatile content in plant tissue and soil samples as well as the associated analytical challenges. Although sensitive analytical methods have been reported in recent decades, studies on their application in modern investigative techniques are lacking. Herein, the latest sampling methods in volatile biochemistry, current advancements in the understanding of these analytes, and the significance of these findings for other types of volatiles are summarized. Gas chromatography, high-performance liquid chromatography, ion chromatography, thin-film microextraction, and real-time monitoring techniques are discussed and critically determined. This review concerns the methods most suitable for future research in this area.

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优化植物组织和土壤样品中挥发性有机化合物的测定：主要活性化合物的非靶向代谢组学研究

本综述严格评估了用不同方法测定低分子量挥发物的情况，为开发合适的技术来测定植物组织和土壤样本中的挥发物含量以及相关的分析挑战提供了背景资料。尽管近几十年来已有敏感分析方法的报道，但缺乏对其在现代调查技术中应用的研究。本文总结了挥发性生物化学的最新取样方法、目前对这些分析物的认识进展以及这些发现对其他类型挥发物的意义。文中讨论了气相色谱法、高效液相色谱法、离子色谱法、薄膜微萃取法和实时监测技术，并对其进行了严格的评判。本综述涉及最适合该领域未来研究的方法。

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