HNRNPA2B1 induces cell proliferation and acts as biomarker in breast cancer.

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS

ACS Applied Bio Materials Pub Date : 2024-01-01 DOI:10.3233/CBM-230576

Yi Yang, Yi Zhang, Tongbao Feng, Chunfu Zhu

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引用次数: 0

Abstract

Background: Numerous studies have shown that m6A plays an important regulatory role in the development of tumors. HNRNPA2B1, one of the m6A RNA methylation reading proteins, has been proven to be elevated in human cancers.

Objective: In this study, we aimed to identify the role of HNRNPA2B1 in breast cancer.

Methods: HNRNPA2B1 expression was investigated via RT-qPCR and TCGA database in breast cancer. Then, the function of HNRNPA2B1 on cancer cell was measured by CCK8 assays, colony formation and scratch assays. In addition, HNRNPA2B1 expression in BRCA was explored via the Wilcoxon signed-rank test, KruskalWallis test and logistic regression. The association with HNRNPA2B1 expression and survival were considered by KaplanMeier and Cox regression analyses. The biological function of HNRNPA2B1 was analyzed via gene set enrichment analysis (GSEA) and the cluster Profiler R software package.

Results: We found that HNRNPA2B1 was highly expressed and induced cell proliferation and migration in breast cancer. Moreover, we observed HNRNPA2B1 induced tumor growth in vivo. In addition, we also found HNRNPA2B1 expression was associated with characteristics and prognosis in breast cancer patients.

Conclusion: Our findings suggested that HNRNPA2B1 promoted tumor growth and could function as a new potential molecular marker in breast cancer.

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HNRNPA2B1 可诱导细胞增殖并作为乳腺癌的生物标志物。

背景：大量研究表明，m6A在肿瘤的发生发展中起着重要的调控作用。HNRNPA2B1 是 m6A RNA 甲基化读取蛋白之一，已被证实在人类癌症中升高：材料与方法：通过 RT-qPCR 和 TCGA 数据库检测乳腺癌中 HNRNPA2B1 的表达。然后，通过 CCK8 试验、集落形成和划痕试验测定 HNRNPA2B1 对癌细胞的作用。此外，还通过 Wilcoxon 符号秩检验、KruskalWallis 检验和逻辑回归探讨了 HNRNPA2B1 在 BRCA 中的表达。通过 KaplanMeier 和 Cox 回归分析考虑了 HNRNPA2B1 表达与生存的关系。通过基因组富集分析（GSEA）和cluster Profiler R软件包分析了HNRNPA2B1的生物学功能：结果：我们发现，HNRNPA2B1在乳腺癌中高表达并诱导细胞增殖和迁移。此外，我们还观察到 HNRNPA2B1 在体内诱导肿瘤生长。此外，我们还发现 HNRNPA2B1 的表达与乳腺癌患者的特征和预后有关：结论：我们的研究结果表明，HNRNPA2B1能促进肿瘤生长，可作为乳腺癌的一种新的潜在分子标记物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Bio Materials Chemistry-Chemistry (all)

CiteScore

9.40

自引率

2.10%

发文量

464