Comparison of susceptibility of Pseudomonas aeruginosa isolated from keratitis to antibiotics and multipurpose disinfecting solutions

IF 4.1 3区医学 Q1 OPHTHALMOLOGY

Contact Lens & Anterior Eye Pub Date : 2024-08-22 DOI:10.1016/j.clae.2024.102288

Motohiro Itoi , Mark D.P. Willcox

{"title":"Comparison of susceptibility of Pseudomonas aeruginosa isolated from keratitis to antibiotics and multipurpose disinfecting solutions","authors":"Motohiro Itoi , Mark D.P. Willcox","doi":"10.1016/j.clae.2024.102288","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><div>This study aimed to assess the susceptibility of ocular isolates of <em>Pseudomonas aeruginosa</em> to two multi-purpose disinfectant solutions (MPDS) and to determine if there was a relationship with resistance to antibiotics.</div></div><div><h3>Methods</h3><div>Twenty-three strains of <em>P. aeruginosa</em> isolated from microbial keratitis cases in Australia were utilized in this study. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of two commercially available MPDSs, Biotrue and cleadew MPS were determined. Additionally, the MIC of the strains to five antibiotics, ciprofloxacin, levofloxacin, gentamicin, ceftazidime, and imipenem, were analyzed.</div></div><div><h3>Results</h3><div>All strains were susceptible to 100 % of each of the MPDS. However, when MPDSs were diluted, cleadew MPS had significantly lower median MIC (median = 12.5 vs 25; p = 0.00008) and MBC (median = 25 vs 50; p = 0.0027) compared to Biotrue. All tested strains were susceptible to levofloxacin and gentamicin. Susceptibility rates to ciprofloxacin, imipenem, and ceftazidime were 52.2 %, 30.4 %, and 91.3 %, respectively. There were no significant relations between MIC or MBC to either MPDS and resistance to the antibiotics (p≧0.23).</div></div><div><h3>Conclusion</h3><div>Both MPDSs were active against <em>P. aeruginosa</em> isolated from microbial keratitis in Australia. However, after dilution, cleadew MPS remained active against <em>P. aeruginosa</em> at lower concentrations. Certain strains of <em>P. aeruginosa</em> were resistant to imipenem, ceftazidime or ciprofloxacin. The lack of association of susceptibility to MPDS and antibiotics suggest that resistance to one did not predispose to resistance to the other.</div></div>","PeriodicalId":49087,"journal":{"name":"Contact Lens & Anterior Eye","volume":"47 6","pages":"Article 102288"},"PeriodicalIF":4.1000,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Contact Lens & Anterior Eye","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1367048424001814","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"OPHTHALMOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose

This study aimed to assess the susceptibility of ocular isolates of Pseudomonas aeruginosa to two multi-purpose disinfectant solutions (MPDS) and to determine if there was a relationship with resistance to antibiotics.

Methods

Twenty-three strains of P. aeruginosa isolated from microbial keratitis cases in Australia were utilized in this study. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of two commercially available MPDSs, Biotrue and cleadew MPS were determined. Additionally, the MIC of the strains to five antibiotics, ciprofloxacin, levofloxacin, gentamicin, ceftazidime, and imipenem, were analyzed.

Results

All strains were susceptible to 100 % of each of the MPDS. However, when MPDSs were diluted, cleadew MPS had significantly lower median MIC (median = 12.5 vs 25; p = 0.00008) and MBC (median = 25 vs 50; p = 0.0027) compared to Biotrue. All tested strains were susceptible to levofloxacin and gentamicin. Susceptibility rates to ciprofloxacin, imipenem, and ceftazidime were 52.2 %, 30.4 %, and 91.3 %, respectively. There were no significant relations between MIC or MBC to either MPDS and resistance to the antibiotics (p≧0.23).

Conclusion

Both MPDSs were active against P. aeruginosa isolated from microbial keratitis in Australia. However, after dilution, cleadew MPS remained active against P. aeruginosa at lower concentrations. Certain strains of P. aeruginosa were resistant to imipenem, ceftazidime or ciprofloxacin. The lack of association of susceptibility to MPDS and antibiotics suggest that resistance to one did not predispose to resistance to the other.

查看原文本刊更多论文

比较从角膜炎中分离出来的铜绿假单胞菌对抗生素和多用途消毒液的敏感性。

目的：本研究旨在评估眼部铜绿假单胞菌分离株对两种多用途消毒剂溶液（MPDS）的敏感性，并确定其是否与抗生素耐药性有关：本研究采用了从澳大利亚微生物角膜炎病例中分离出的 23 株铜绿假单胞菌。测定了两种市售 MPDS（Biotrue 和 cleadew MPS）的最低抑菌浓度 (MIC) 和最低杀菌浓度 (MBC)。此外，还分析了菌株对环丙沙星、左氧氟沙星、庆大霉素、头孢他啶和亚胺培南这五种抗生素的 MIC：结果：所有菌株对每种 MPDS 的敏感率均为 100%。然而，当稀释 MPDS 时，与 Biotrue 相比，cleadew MPS 的 MIC 中位数（中位数 = 12.5 vs 25；p = 0.00008）和 MBC 中位数（中位数 = 25 vs 50；p = 0.0027）明显较低。所有测试菌株都对左氧氟沙星和庆大霉素敏感。对环丙沙星、亚胺培南和头孢他啶的敏感率分别为 52.2%、30.4% 和 91.3%。两种多肽释放抑制剂的 MIC 或 MBC 与抗生素耐药性之间无明显关系（p≧0.23）：结论：两种 MPDS 对从澳大利亚微生物性角膜炎中分离出的铜绿假单胞菌都有活性。然而，稀释后，在较低浓度下，洁霉素 MPS 对铜绿假单胞菌仍有活性。某些铜绿假单胞菌菌株对亚胺培南、头孢他啶或环丙沙星具有抗药性。对 MPDS 和抗生素的敏感性没有关联，这表明对其中一种抗生素的耐药性不会导致对另一种抗生素的耐药性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Contact Lens & Anterior Eye OPHTHALMOLOGY-

CiteScore

7.60

自引率

18.80%

发文量

198

审稿时长

55 days

期刊介绍： Contact Lens & Anterior Eye is a research-based journal covering all aspects of contact lens theory and practice, including original articles on invention and innovations, as well as the regular features of: Case Reports; Literary Reviews; Editorials; Instrumentation and Techniques and Dates of Professional Meetings.