Emergence of the mobile colistin resistance gene mcr-1 in a Leclercia adecarboxylata strain isolated from wastewater in Seoul

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES
Jin Seok Kim, Hyo-Won Jeong, Young Hee Jin, JinWoo Kim, Jin-ah Kim, Sook Hyun Park, So-Mi Yoon, Sang-Eun Jung, Jung Im Jang, Eun Ji Kim, Jae In Lee, Jib-Ho Lee
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Abstract

Colistin is considered the last resort for treating infections caused by multidrug-resistant bacteria. However, the spread of the plasmid-borne colistin-resistance gene mcr-1 has become a public health threat. In this study, we identified mcr-1-harboring Leclercia adecarboxylata strain (WWCOL-134) isolated from wastewater in Seoul. The strain had a colistin MIC value of 2 µg/ml and was resistant to cefotaxime, gentamicin, tetracycline, trimethoprim and sulfamethoxazole. The mcr-1 gene, along with an array of resistance genes, was located on a 236-kb plasmid (pCOL134-1), which contained the typical IncHI2 backbone of reported mcr-1-carrying plasmids, and was transferred to an Escherichia coli strain by conjugation. To the best of our knowledge, this is the first study to report the emergence of mcr-1-harboring Leclercia sp. isolate. Our findings demonstrate the ongoing spread of colistin resistance among Enterobacterales species, emphasizing the need for surveillance of antimicrobial resistance in wastewater environments.

从首尔废水中分离出的一种 adecarboxylata Leclercia 菌株中出现了可移动的可乐定抗性基因 mcr-1。
可乐定被认为是治疗耐多药细菌感染的最后手段。然而,质粒携带的耐秋水仙碱基因 mcr-1 的传播已成为一种公共卫生威胁。在本研究中,我们发现了从首尔废水中分离出的携带 mcr-1 的 Leclercia adecarboxylata 菌株(WWCOL-134)。该菌株对可乐菌素的 MIC 值为 2 µg/ml,对头孢他啶、庆大霉素、四环素、三甲双胍和磺胺甲噁唑有抗药性。mcr-1 基因和一系列抗性基因位于一个 236 kb 的质粒(pCOL134-1)上,该质粒含有已报道的携带 mcr-1 的质粒的典型 IncHI2 主干,并通过共轭转入大肠杆菌菌株。据我们所知,这是首次报道出现携带 mcr-1 的大肠杆菌分离物的研究。我们的研究结果表明,肠杆菌科细菌对可乐定的耐药性正在不断扩散,这强调了对废水环境中抗菌素耐药性进行监控的必要性。
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来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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