Identification of Two HIV-1 CRF01_AE/B Recombinant Forms and a CRF01_AE/B/C Recombinant Form in Hebei Province, China.

IF 0.8 4区医学 Q4 IMMUNOLOGY

Current HIV Research Pub Date : 2024-01-01 DOI:10.2174/011570162X294091240812062836

Xiangyun Tang, Meng Liu, Ning An, Xinyu Zhang, Yingying Wang, Yan Li, Xinli Lu

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引用次数: 0

Abstract

Background: In the Hebei province, Human Immunodeficiency Virus type one (HIV-1) recombinant strains of subtypes B, C, and CRF01_AE are emerging very rapidly and diversely.

Objective: In order to confirm the characteristics of novel recombination forms, we aimed to analyze HIV-1 Near-full-length Genome sequences (NFLGs) obtained from three Men who have Sex with Men (MSM) in this study.

Methods: Phylogenetic trees were constructed and breakpoints analysis were performed based on the NFLGs and each gene fragment to examine the gene recombination patterns of three new HIV-1 NFLGs.

Results: HIV-1 subtypes CRF01_AE and B were combined to generate the recombinant structures of the NFLGs 610 and 687. CRF01_AE, B, and C were combined to generate the recombinant structures of the NFLG 825. According to the NFLG phylogenetic tree, the NFLG 825 clustered with CRF65_cpx and the NFLGs 610 and 687 clustered with CRF68_01B. The recombination breakpoints analysis revealed that the recombination pattern of the NFLGs 610 and 687 was the insertion of subtype B fragment into the CRF01_AE backbone. Subregions I, II, and III were derived from CRF01_AE, subtype B, and CRF01_AE, respectively. The recombination pattern of the NFLG 825 contained ten fragments of subtypes CRF01_AE, C, and B. Finally, the above factors were formed using phylogenetic trees and breakpoints analysis, which were combined to get two CRF68_01B forms and one CRF65_cpx form.

Conclusion: Our findings have suggested that it is crucial to keep an eye on the genetic diversity of HIV-1 in Hebei province.

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在中国河北省鉴定出两种 HIV-1 CRF01_AE/B 重组形式和一种 CRF01_AE/B/C 重组形式。

背景在河北省，B、C和CRF01_AE亚型的人类免疫缺陷病毒（HIV-1）重组株的出现非常迅速且多样化：为了确认新型重组形式的特征，我们在本研究中分析了从三名男男性行为者（MSM）身上获得的 HIV-1 近全长基因组序列（NFLGs）：方法：构建系统发生树，并根据 NFLGs 和每个基因片段进行断点分析，研究三种新 HIV-1 NFLGs 的基因重组模式：结果：HIV-1亚型CRF01_AE和B结合产生了NFLGs 610和687的重组结构。CRF01_AE、B 和 C 结合生成了 NFLG 825 的重组结构。根据 NFLG 系统发生树，NFLG 825 与 CRF65_cpx 聚类，NFLG 610 和 687 与 CRF68_01B 聚类。重组断点分析表明，NFLGs 610 和 687 的重组模式是将 B 亚型片段插入 CRF01_AE 主干。亚区 I、II 和 III 分别来自 CRF01_AE、B 亚型和 CRF01_AE。NFLG 825的重组模式包含了CRF01_AE、C和B亚型的10个片段。最后，利用系统发生树和断点分析法形成了上述因子，并将其合并得到了两个CRF68_01B形式和一个CRF65_cpx形式：我们的研究结果表明，关注河北省 HIV-1 遗传多样性至关重要。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current HIV Research 医学-病毒学

CiteScore

1.90

自引率

10.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Current HIV Research covers all the latest and outstanding developments of HIV research by publishing original research, review articles and guest edited thematic issues. The novel pioneering work in the basic and clinical fields on all areas of HIV research covers: virus replication and gene expression, HIV assembly, virus-cell interaction, viral pathogenesis, epidemiology and transmission, anti-retroviral therapy and adherence, drug discovery, the latest developments in HIV/AIDS vaccines and animal models, mechanisms and interactions with AIDS related diseases, social and public health issues related to HIV disease, and prevention of viral infection. Periodically, the journal invites guest editors to devote an issue on a particular area of HIV research of great interest that increases our understanding of the virus and its complex interaction with the host.