Molecular mechanisms regulating glucose metabolism in quinoa (Chenopodium quinoa Willd.) seeds under drought stress.

IF 5.4 3区材料科学 Q2 CHEMISTRY, PHYSICAL

ACS Applied Energy Materials Pub Date : 2024-08-23 DOI:10.1186/s12870-024-05510-w

Chunmei Wang, Chuan Lu, Junling Wang, Xiaoqing Liu, Zhimin Wei, Yan Qin, Huilong Zhang, Xiaoxia Wang, Boxiang Wei, Wei Lv, Guojun Mu

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引用次数: 0

Abstract

Background: Abiotic stress seriously affects the growth and yield of crops. It is necessary to search and utilize novel abiotic stress resistant genes for 2.0 breeding programme in quinoa. In this study, the impact of drought stress on glucose metabolism were investigated through transcriptomic and metabolomic analyses in quinoa seeds. Candidate drought tolerance genes on glucose metabolism pathway were verified by qRT-PCR combined with yeast expression system.

Results: From 70 quinoa germplasms, drought tolerant material M059 and drought sensitive material M024 were selected by comprehensive evaluation of drought resistance. 7042 differentially expressed genes (DEGs) were indentified through transcriptomic analyses. Gene Ontology (GO) analysis revealed that these DEGs were closely related to carbohydrate metabolic process, phosphorus-containing groups, and intracellular membrane-bounded organelles. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis detected that DEGs were related to pathways involving carbohydrate metabolisms, glycolysis and gluconeogenesis. Twelve key differentially accumulated metabolites (DAMs), (D-galactose, UDP-glucose, succinate, inositol, D-galactose, D-fructose-6-phosphate, D-glucose-6-phosphate, D-glucose-1-phosphate, dihydroxyacetone phosphate, ribulose-5-phosphate, citric acid and L-malate), and ten key candidate DEGs (CqAGAL2, CqINV, CqFrK7, CqCELB, Cqbg1x, CqFBP, CqALDO, CqPGM, CqIDH3, and CqSDH) involved in drought response were identified. CqSDH, CqAGAL2, and Cqβ-GAL13 were candidate genes that have been validated in both transcriptomics and yeast expression screen system.

Conclusion: These findings provide a foundation for elucidating the molecular regulatory mechanisms governing glucose metabolism in quinoa seeds under drought stress, providing insights for future research exploring responses to drought stress in quinoa.

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干旱胁迫下藜麦种子葡萄糖代谢的分子调控机制

背景：非生物胁迫严重影响作物的生长和产量。在藜麦 2.0 育种计划中，有必要寻找和利用新型抗非生物胁迫基因。本研究通过对藜麦种子的转录组和代谢组分析，研究了干旱胁迫对葡萄糖代谢的影响。结合酵母表达系统，通过 qRT-PCR 验证了葡萄糖代谢途径上的候选耐旱基因：结果：通过抗旱性综合评价，从 70 个藜麦种质资源中筛选出耐旱材料 M059 和干旱敏感材料 M024。通过转录组分析确定了 7042 个差异表达基因（DEGs）。基因本体（Gene Ontology，GO）分析表明，这些差异表达基因与碳水化合物代谢过程、含磷基团和细胞内膜束细胞器密切相关。京都基因和基因组百科全书》（KEGG）富集分析发现，DEGs 与涉及碳水化合物代谢、糖酵解和葡萄糖生成的通路有关。CqAGAL2、CqINV、CqFrK7、CqCELB、Cqbg1x、CqFBP、CqALDO、CqPGM、CqIDH3 和 CqSDH），以及参与干旱响应的 10 个关键候选 DEGs（CqAGAL2、CqINV、CqFrK7、CqCELB、Cqbg1x、CqFBP、CqALDO、CqPGM、CqIDH3 和 CqSDH）。CqSDH、CqAGAL2和Cqβ-GAL13是在转录组学和酵母表达筛选系统中得到验证的候选基因：这些发现为阐明干旱胁迫下藜麦种子葡萄糖代谢的分子调控机制奠定了基础，为今后探索藜麦干旱胁迫响应的研究提供了启示。

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来源期刊

ACS Applied Energy Materials Materials Science-Materials Chemistry

CiteScore

10.30

自引率

6.20%

发文量

1368

期刊介绍： ACS Applied Energy Materials is an interdisciplinary journal publishing original research covering all aspects of materials, engineering, chemistry, physics and biology relevant to energy conversion and storage. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important energy applications.