Spatial Activation of Autophagy in Human Placenta-Related Tissue During Labor: A Possible Mechanism for Labor Onset

IF 2.5 3区医学 Q3 IMMUNOLOGY

American Journal of Reproductive Immunology Pub Date : 2024-08-23 DOI:10.1111/aji.13903

Jing Shang, Zhijian Wang, Yingying Huang, Yuyu Wu, Jin Jin

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引用次数: 0

Abstract

Introduction

To explore the mechanisms of labor by investigating the autophagy of placental and fetal membranes tissue in normal pregnant women.

Methods

Placenta and fetal membranes were collected from women with singleton pregnancies without any medical complications and from women who delivered vaginally (labor-initiated group; L group) or by caesarean section (labor-noninitiated group; NL group). Autophagosomes were observed by transmission electron microscopy (TEM). Immunofluorescence and western blotting (WB) were used to detect protein levels of the autophagy markers LC3A and LC3B. TEM, immunohistochemistry (IHC), and WB were used to compare autophagy in different parts of the placenta and fetal membranes in the L and NL groups. The expression of LC3B/LC3A, ROCK1, and ROCK2 in the placenta of nonpregnant and pregnant rats was detected by WB and IHC.

Results

TEM and IHC results showed an increase in the number of autophagosomes and autophagic lysosomes in the L group, and WB results indicated an increase in the LC3B/A ratio between the placenta and fetal membranes in the L group. Autophagy was significantly increased on the maternal side of the placenta in the L group, and the level of autophagy became higher near rupture in the fetal membranes and near the point where the umbilical cord joins the placenta in the L group. The LC3B/A ratio increased and ROCK1 and ROCK2 levels decreased in postnatal rats.

Discussion

Autophagy can occur in the placenta and fetal membranes and its activity is higher at the onset of labor, suggesting a role in labor.

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分娩过程中人类胎盘相关组织自噬的空间激活：分娩开始的可能机制

引言通过研究正常孕妇胎盘和胎膜组织的自噬现象，探讨分娩机制：方法：从无任何并发症的单胎妊娠妇女和经阴道分娩（Labor-initiated 组；L 组）或剖腹产（Labor-non-initiated 组；NL 组）的妇女中收集胎盘和胎膜。通过透射电子显微镜（TEM）观察自噬体。免疫荧光和免疫印迹（WB）用于检测自噬标记物 LC3A 和 LC3B 的蛋白水平。利用TEM、免疫组织化学（IHC）和WB比较L组和NL组胎盘和胎膜不同部位的自噬情况。通过 WB 和 IHC 检测未孕大鼠和妊娠大鼠胎盘中 LC3B/LC3A、ROCK1 和 ROCK2 的表达：结果：TEM和IHC结果显示L组自噬体和自噬溶酶体数量增加，WB结果显示L组胎盘和胎膜的LC3B/A比值增加。L 组胎盘母体一侧的自噬明显增加，L 组胎膜破裂附近和脐带与胎盘连接点附近的自噬水平更高。LC3B/A比值升高，ROCK1和ROCK2水平降低：自噬可发生在胎盘和胎膜中，其活性在分娩开始时更高，这表明自噬在分娩中发挥作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American Journal of Reproductive Immunology 医学-免疫学

CiteScore

6.20

自引率

5.60%

发文量

314

审稿时长

2 months

期刊介绍： The American Journal of Reproductive Immunology is an international journal devoted to the presentation of current information in all areas relating to Reproductive Immunology. The journal is directed toward both the basic scientist and the clinician, covering the whole process of reproduction as affected by immunological processes. The journal covers a variety of subspecialty topics, including fertility immunology, pregnancy immunology, immunogenetics, mucosal immunology, immunocontraception, endometriosis, abortion, tumor immunology of the reproductive tract, autoantibodies, infectious disease of the reproductive tract, and technical news.