Recurrent cervical cancer detection using DNA methylation markers in self-collected samples from home.

IF 5.7 2区 医学 Q1 ONCOLOGY
International Journal of Cancer Pub Date : 2025-02-01 Epub Date: 2024-08-22 DOI:10.1002/ijc.35143
Mirte Schaafsma, Rianne van den Helder, Constantijne H Mom, Renske D M Steenbergen, Maaike C G Bleeker, Nienke E van Trommel
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引用次数: 0

Abstract

Early detection of recurrent cervical cancer is important to improve survival rates. The aim of this study was to explore the clinical performance of DNA methylation markers and high-risk human papillomavirus (HPV) in cervicovaginal self-samples and urine for the detection of recurrent cervical cancer. Cervical cancer patients without recurrence (n = 47) collected cervicovaginal self-samples and urine pre- and posttreatment. Additionally, 20 patients with recurrent cervical cancer collected cervicovaginal self-samples and urine at time of recurrence. All samples were self-collected at home and tested for DNA methylation and high-risk HPV DNA by PCR. In patients without recurrent cervical cancer, DNA methylation levels decreased 2-years posttreatment compared to pretreatment in cervicovaginal self-samples (p < .0001) and urine (p < .0001). DNA methylation positivity in cervicovaginal self-samples was more frequently observed in patients with recurrence (77.8%) than in patients without recurrence 2-years posttreatment (25.5%; p = .0004). Also in urine, DNA methylation positivity was more frequently observed in patients with recurrence (65%) compared to those without recurrence (35.6%; p = .038). Similarly, high-risk HPV positivity in both cervicovaginal self-samples and urine was more frequent (52.6% and 55%, respectively) in patients with recurrence compared to patients without recurrence (14.9% and 8.5%, respectively) (p = .004 and p = .0001). In conclusion, this study shows the potential of posttreatment monitoring of cervical cancer patients for recurrence by DNA methylation and high-risk HPV testing in cervicovaginal and urine samples collected at home. The highest recurrence detection rate was achieved by DNA methylation testing in cervicovaginal self-samples, detecting 77.8% of all recurrences and, specifically, 100% of the local recurrences.

利用家庭自采样本中的 DNA 甲基化标记检测复发性宫颈癌。
早期发现复发性宫颈癌对提高生存率非常重要。本研究旨在探讨宫颈阴道自取样本和尿液中的 DNA 甲基化标记物和高危人乳头瘤病毒(HPV)在检测复发性宫颈癌方面的临床表现。未复发的宫颈癌患者(47 人)在治疗前后采集了宫颈阴道自检样本和尿液。此外,20 名复发宫颈癌患者在复发时采集了宫颈阴道自采样本和尿液。所有样本均在家中自行采集,并通过 PCR 检测 DNA 甲基化和高危 HPV DNA。在没有复发宫颈癌的患者中,治疗后 2 年的宫颈阴道自取样本中的 DNA 甲基化水平与治疗前相比有所下降(p
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来源期刊
CiteScore
13.40
自引率
3.10%
发文量
460
审稿时长
2 months
期刊介绍: The International Journal of Cancer (IJC) is the official journal of the Union for International Cancer Control—UICC; it appears twice a month. IJC invites submission of manuscripts under a broad scope of topics relevant to experimental and clinical cancer research and publishes original Research Articles and Short Reports under the following categories: -Cancer Epidemiology- Cancer Genetics and Epigenetics- Infectious Causes of Cancer- Innovative Tools and Methods- Molecular Cancer Biology- Tumor Immunology and Microenvironment- Tumor Markers and Signatures- Cancer Therapy and Prevention
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