Insecticidal activities of three recombinant venom proteins of the predatory stink bug, Arma custos

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2024-08-21 DOI:10.1002/ps.8382

Xinyi Wang, Wenhong Li, Xiang Yang, Mingwei Yang, Yucheng Gu, Zhao Du, Jingyi Yang, Mingxia Wen, Yoonseong Park, Chunyang Huang, Yueping He

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引用次数: 0

Abstract

BACKGROUND

Widespread resistance of insect pests to insecticides and transgenic crops in the field is a significant challenge for sustainable agriculture, and calls for the development of novel alternative strategies to control insect pests. One potential resource for the discovery of novel insecticidal molecules is natural toxins, particularly those derived from the venoms of insect predators.

RESULTS

In this study, we identified three insecticidal proteinaceous toxins from the venom glands (VGs) of the predatory stink bug, Arma custos (Hemiptera: Asopinae). Transcriptomic analysis of A. custos VGs revealed 151 potentially secreted VG-rich venom proteins. Three VG-rich venom proteins (designated AcVP1 ~ 3) were produced by overexpression in Escherichia coli. Injection of the recombinant proteins into tobacco cutworm (Spodoptera litura) larvae showed that all of the three recombinant proteins caused paralysis, liquefaction and death. Injection of recombinant proteins into rice brown planthopper (Nilaparvata lugens) nymphs showed higher insecticidal activities, among which a trypsin (AcVP2) caused 100% mortality postinjection at 1.27 pmol mg⁻¹ body weight.

CONCLUSION

查看原文本刊更多论文

捕食蝽三种重组毒液蛋白的杀虫活性。

背景：田间害虫对杀虫剂和转基因作物的广泛抗药性是可持续农业面临的一个重大挑战，这就要求开发新的替代战略来控制害虫。发现新型杀虫分子的一个潜在资源是天然毒素，特别是从昆虫天敌毒液中提取的毒素：结果：在这项研究中，我们从捕食性蝽类 Arma custos（半翅目：疟原虫科）的毒腺（VGs）中鉴定出了三种杀虫蛋白类毒素。库斯托斯毒腺的转录组分析发现了 151 种潜在的富含毒腺分泌物的毒液蛋白。通过在大肠杆菌中过表达，产生了三种富含 VG 的毒液蛋白（命名为 AcVP1 ~ 3）。将重组蛋白注射到烟草切割虫（Spodoptera litura）幼虫体内后发现，这三种重组蛋白都能导致幼虫麻痹、液化和死亡。将重组蛋白注射到水稻褐飞虱若虫体内显示出较高的杀虫活性，其中一种胰蛋白酶（AcVP2）在注射1.27 pmol mg-1体重后可导致100%死亡：结论：本研究揭示了从捕食性昆虫中发现杀虫毒素的天然工具包。© 2024 化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.