Assessment of Sex, Gonad Volume, and Reproductive Maturation Status of the Indian Shad, Tenualosa ilisha, Using Ultrasonic Imaging: A Rapid and Non-Invasive Tool

IF 0.7 4区 农林科学 Q4 FISHERIES
Subrata Dasgupta, Gouranga Biswas, Gayatri Tripathi, Mujahidkhan A. Pathan, Tapas K. Ghoshal, Pratiksha K. Singh, Tanmoy Jana, Paroma Mitra, Srikanta Samanta, Debasis De, Subhendu Adhikari, Ranjan K. Manna, Amiya K. Sahoo
{"title":"Assessment of Sex, Gonad Volume, and Reproductive Maturation Status of the Indian Shad, Tenualosa ilisha, Using Ultrasonic Imaging: A Rapid and Non-Invasive Tool","authors":"Subrata Dasgupta,&nbsp;Gouranga Biswas,&nbsp;Gayatri Tripathi,&nbsp;Mujahidkhan A. Pathan,&nbsp;Tapas K. Ghoshal,&nbsp;Pratiksha K. Singh,&nbsp;Tanmoy Jana,&nbsp;Paroma Mitra,&nbsp;Srikanta Samanta,&nbsp;Debasis De,&nbsp;Subhendu Adhikari,&nbsp;Ranjan K. Manna,&nbsp;Amiya K. Sahoo","doi":"10.1155/2024/6597804","DOIUrl":null,"url":null,"abstract":"<div>\n <p>To determine sex and maturity stages accurately without any physical injury and stress, especially for sensitive and high-value fish species, employing a noninvasive ultrasound imaging technique could be a desirable approach. The ultrasound imaging method as a powerful eco-friendly tool was established to determine sex, gonad volume, gonadosomatic index (GSI), and reproductive stages in the Indian shad, hilsa (<i>Tenualosa ilisha</i>). About 30 hilsa (15 males and 15 females) of different maturity stages were collected from the river Ganga round the year using gill nets. The ultrasound sonography (USG) was then employed in hilsa to determine the computed GSI. The fresh gonad volume of hilsa was determined using a water displacement method to ascertain actual GSI values. There was no significant difference between the calculated, real, and actual GSIs (<i>P</i> &gt; 0.05) in both males and females. The validation of the precise maturity stages of ultrasound images of the gonad by the histological architecture indicated that USG images of the hilsa gonad depicted exact stages of maturity in both sexes except for stages I and II in males. The sex of the fish was accurately ascertained using ovarian ultrasound scanning for all the specimens. The calculated USG-based ovarian volume was positively correlated (<i>R</i><sup>2</sup> = 0.97) with the actual and real ovarian volumes. The noninvasive and reliable ultrasonography technique was found to be an accurate and valid tool to track gender and gonadal development and predict the spawning periodicity in hilsa.</p>\n </div>","PeriodicalId":14894,"journal":{"name":"Journal of Applied Ichthyology","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1155/2024/6597804","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Ichthyology","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1155/2024/6597804","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0

Abstract

To determine sex and maturity stages accurately without any physical injury and stress, especially for sensitive and high-value fish species, employing a noninvasive ultrasound imaging technique could be a desirable approach. The ultrasound imaging method as a powerful eco-friendly tool was established to determine sex, gonad volume, gonadosomatic index (GSI), and reproductive stages in the Indian shad, hilsa (Tenualosa ilisha). About 30 hilsa (15 males and 15 females) of different maturity stages were collected from the river Ganga round the year using gill nets. The ultrasound sonography (USG) was then employed in hilsa to determine the computed GSI. The fresh gonad volume of hilsa was determined using a water displacement method to ascertain actual GSI values. There was no significant difference between the calculated, real, and actual GSIs (P > 0.05) in both males and females. The validation of the precise maturity stages of ultrasound images of the gonad by the histological architecture indicated that USG images of the hilsa gonad depicted exact stages of maturity in both sexes except for stages I and II in males. The sex of the fish was accurately ascertained using ovarian ultrasound scanning for all the specimens. The calculated USG-based ovarian volume was positively correlated (R2 = 0.97) with the actual and real ovarian volumes. The noninvasive and reliable ultrasonography technique was found to be an accurate and valid tool to track gender and gonadal development and predict the spawning periodicity in hilsa.

Abstract Image

利用超声波成像评估印度鲥鱼(Tenualosa ilisha)的性别、性腺体积和生殖成熟状态:快速、非侵入性工具
为了在不造成任何身体伤害和压力的情况下准确测定鱼类的性别和成熟阶段,尤其是敏感和高价值鱼类,采用无创超声波成像技术不失为一种可取的方法。超声波成像方法是一种强大的生态友好型工具,用于确定印度鲥鱼(Tenualosa ilisha)的性别、性腺体积、性腺指数(GSI)和生殖阶段。使用刺网从恒河中全年收集了约 30 条不同成熟阶段的印度鲥鱼(雌雄各 15 条)。然后采用超声波声学造影(USG)测定鲥鱼的计算GSI。采用水位移法测定希尔沙鱼的新鲜生殖腺体积,以确定实际的 GSI 值。雄性和雌性的计算 GSI 值、实际 GSI 值和实际 GSI 值之间没有明显差异(P > 0.05)。通过组织学结构验证性腺超声波图像的精确成熟阶段表明,除了雄鱼的 I 期和 II 期外,雌雄鱼的性腺 USG 图像都描述了精确的成熟阶段。所有标本的卵巢超声扫描都能准确确定鱼的性别。基于 USG 计算的卵巢体积与实际和真实卵巢体积呈正相关(R2 = 0.97)。研究发现,无创、可靠的超声波成像技术是追踪希尔沙鱼性别、性腺发育和预测产卵周期的准确、有效的工具。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Applied Ichthyology
Journal of Applied Ichthyology 生物-海洋与淡水生物学
CiteScore
2.30
自引率
11.10%
发文量
73
审稿时长
3-8 weeks
期刊介绍: The Journal of Applied Ichthyology publishes articles of international repute on ichthyology, aquaculture, and marine fisheries; ichthyopathology and ichthyoimmunology; environmental toxicology using fishes as test organisms; basic research on fishery management; and aspects of integrated coastal zone management in relation to fisheries and aquaculture. Emphasis is placed on the application of scientific research findings, while special consideration is given to ichthyological problems occurring in developing countries. Article formats include original articles, review articles, short communications and technical reports.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信