First Molecular Evidence of Leishmania Infantum in Domestic Cats and Associated Risk Factors from the Black Sea Region of Türkiye

IF 1.2 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2024-08-20 DOI:10.1007/s11686-024-00885-0

Didem Pekmezci, Alparslan Yildirim, Zeynep Nurselin Kot, Gamze Nur Konacoglu, Onder Duzlu, Gamze Yetismis, Sadullah Uslu, Seray Toz, Yusuf Ozbel, Abdullah Inci, Gokmen Zafer Pekmezci

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引用次数: 0

Abstract

Purpose

The objectives of the present study are to determine the molecular prevalence of Leishmania spp. in the owned domestic cats in the Black Sea Region of Türkiye and analyze the associated risk factors in FeL.

Methods

Conjunctival swabs (CS), blood, demographic, and clinical data were collected from 150 owned cats brought to the Veterinary Teaching Hospital during 2020–2022. Leishmania kinetoplast DNA (kDNA) from CS was screened by TaqMan Real-Time PCR (qPCR) with the genus-specific primers and a probe.

Results

All qPCR positive products were also amplified and sequenced to identify Leishmania species by ITS1 primers. Molecular prevalence of L. infantum found as 12.6% (19/150) in the observed cats in the Black Sea Region of Türkiye. There was a significant difference (p < 0.05) between neutered and intact cats with regarding to L. infantum positivity. Intact cats found to be 0.368 times more prone to be L. infantum-positive (L+). Dermatological lesions were found the most common (26.3%) problems in the L + cats. The median leucocyte count was the only parameter that was found statistically (p < 0.05) lower in the L + group (6.60) than the negative group (L−) (8.96), when comparing the WBC, NEU/LYM, MONO/LYM, EOS/LYM and PLT/LYM values.

Conclusion

This study presented the molecular occurrence of FeL in the Black Sea Region of Türkiye for the first time indicating that the carrier status of the cats makes them alternative reservoirs for possible zoonotic transmission of L. infantum in this zone.

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图尔基耶黑海地区家猫幼年利什曼病及其相关风险因素的首个分子证据。

目的：本研究的目的是确定利什曼原虫在土耳其黑海地区饲养的家猫中的分子流行率，并分析FeL的相关风险因素：方法：收集了 2020-2022 年期间送到兽医教学医院的 150 只家猫的结膜拭子 (CS)、血液、人口统计学和临床数据。利用属特异性引物和探针，通过 TaqMan 实时定量 PCR（qPCR）对 CS 中的利什曼原虫 DNA（kDNA）进行筛选：结果：所有 qPCR 阳性产物均通过 ITS1 引物进行扩增和测序，以确定利什曼病种。在土耳其黑海地区观察到的猫中，婴儿利什曼原虫的分子流行率为 12.6%（19/150）。两者之间存在明显差异（p 结论）：本研究首次揭示了在土耳其黑海地区发生的非洲猪瘟分子病例，表明猫的携带者身份使其成为该地区可能发生的婴儿型非洲猪瘟人畜共患病传播的替代贮藏库。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.