Combining Killed Vaccine Candidate with Different Adjuvants to Determine Prophylactic Potential against Leishmaniasis

IF 1.2 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2024-08-20 DOI:10.1007/s11686-024-00903-1

Kübra Kelleci, Adil Allahverdiyev, Melahat Bağırova, Murat Ihlamur, Emrah Şefik Abamor

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Abstract

Visceral Leishmaniasis is a serious public health problem caused by Leishmania species parasites. Approximately 500 thousand people get Visceral Leishmaniasis (VL) every year. An effective and reliable vaccine against the disease has still not been formulated. Choosing the right adjuvant is important to increase immunogenicity in vaccines prepared with total antigens. In this study, we investigate the ideal adjuvant for use in vaccine formulations against VL. For this purpose, Leishmania antigens (FTLA) obtained from L. infantum parasites by the freeze-thaw method and three different adjuvants (alum-saponin and calcium phosphate) were used. The effectiveness of the formulations was investigated in vitro by cell viability analysis and determination of nitric oxide and cytokine production abilities in J774 macrophage cells. According to the study results, it was determined that formulations prepared with calcium phosphate produced 72% more NO and approximately 7.2 times more IL-12 cytokine. The results obtained showed that calcium phosphate salts can be used as ideal adjuvants in vaccine research against leishmaniasis.

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将候选杀灭疫苗与不同的佐剂相结合，以确定其对利什曼病的预防潜力。

皮损利什曼病是由利什曼原虫引起的严重公共卫生问题。每年约有 50 万人感染内脏利什曼病（VL）。目前尚未研制出有效、可靠的疫苗来预防该疾病。选择合适的佐剂对于提高全抗原疫苗的免疫原性非常重要。在这项研究中，我们探讨了用于 VL 疫苗制剂的理想佐剂。为此，我们使用了通过冻融法从幼年利什曼病寄生虫中获得的利什曼病抗原（FTLA）和三种不同的佐剂（明矾-皂素和磷酸钙）。通过细胞活力分析和 J774 巨噬细胞中一氧化氮和细胞因子生成能力的测定，在体外研究了这些制剂的有效性。研究结果表明，用磷酸钙制备的制剂产生的一氧化氮比普通制剂多 72%，产生的 IL-12 细胞因子比普通制剂多约 7.2 倍。研究结果表明，磷酸钙盐可作为理想的佐剂用于利什曼病疫苗研究。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.