Ginsenoside Rb1 ameliorates apical periodontitis via suppressing macrophage pyroptosis.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2025-02-01 Epub Date: 2024-08-18 DOI:10.1111/odi.15103

Xiaoyue Guan, Rui Zhao, Yuting Wang, Wenlan Li, Lifei Pan, Yao Yang, Wenli Mu, Tie Zhou Hou

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引用次数: 0

Abstract

Objectives: The objectives of current study were to investigate the role and related mechanism of Ginsenoside Rb1 (GRb1) on regulating apical periodontitis (AP) prognosis.

Materials and methods: Clinical specimens were used to determine the involvement of calcium overload-induced macrophage pyroptosis in periapical tissues. Next, a calcium ion-chelating agent (BAPTA-AM) was applied to detect the suppression of intracellular calcium overload in macrophage pyroptosis. Then, network pharmacology, western blot (WB) analysis, and Fluo-4 calcium assay were conducted to explore the role of GRb1 on intracellular calcium overload. To gain a better understanding of GRb1 in calcium overload-induced macrophage pyroptosis linked AP, GRb1-treated AP models were established.

Results: We discovered clinically and experimentally that calcium overload-dependent macrophage pyroptosis is involved in AP pathogenesis, and reducing calcium overload greatly decreased macrophage pyroptosis in an AP cell model. Next, based on GRb1's inhibitory role in aberrant intracellular calcium accumulation, we discovered that GRb1 alleviates AP by suppressing calcium-dependent macrophage pyroptosis in both in vitro and in vivo models.

Conclusions: GRb1 is an effective therapeutic strategy to rescue the periapical tissues from inflammation due to its anti-pyroptosis function. Thus, the present study supports further investigation of GRb1 as an adjuvant therapy for AP.

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人参皂苷 Rb1 通过抑制巨噬细胞的嗜热作用改善根尖牙周炎。

研究目的本研究旨在探讨人参皂苷Rb1（GRb1）对根尖周炎（AP）预后的调节作用及相关机制：临床标本用于确定钙超载诱导的巨噬细胞热解在根尖周炎组织中的参与。然后，应用钙离子螯合剂（BAPTA-AM）检测细胞内钙超载对巨噬细胞脓毒症的抑制作用。然后，通过网络药理学、Western blot (WB)分析和Fluo-4钙测定来探讨GRb1对细胞内钙超载的作用。为了更好地了解 GRb1 在钙超载诱导的巨噬细胞脓毒症相关 AP 中的作用，我们建立了 GRb1 处理的 AP 模型：结果：我们通过临床和实验发现，钙超载依赖性巨噬细胞脓毒症参与了AP的发病机制，而在AP细胞模型中，降低钙超载可大大降低巨噬细胞脓毒症的发生。接下来，基于GRb1对细胞内钙异常积累的抑制作用，我们发现GRb1在体外和体内模型中都能通过抑制钙依赖性巨噬细胞脓毒症来缓解AP：结论：GRb1 具有抗脓毒症的功能，是一种有效的治疗策略，可挽救根尖周炎组织。因此，本研究支持将 GRb1 作为 AP 的辅助疗法进行进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.