Bazedoxifene Inhibits Cell Viability, Colony-Forming Activity, and Cell Migration in Human Non–Small Cell Lung Cancer Cells and Improves the Treatment Efficacy of Paclitaxel and Gemcitabine

IF 1.9 4区医学 Q3 RESPIRATORY SYSTEM

Clinical Respiratory Journal Pub Date : 2024-08-17 DOI:10.1111/crj.13822

Yaochen Huang, Jiayuh Lin, Xiangning Fu, Lequn Li, Shenging Fu

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Abstract

Background

Bazedoxifene is a third-generation selective estrogen receptor modulator that inhibits the IL6/IL6R/GP130 signaling pathway by inhibiting IL6-induced homodimerization of GP130. Considering that the IL6/IL6R/GP130 signaling pathway is important in tumorigenesis and metastasis, bazedoxifene is thought to have an antitumor effect, which has been proven preliminarily in breast cancer and pancreatic cancer but has not yet been studied in non–small cell lung cancer (NSCLC). This study is aimed at evaluating the antitumor effect of bazedoxifene in NSCLC.

Methods

A549 and H1299 NSCLC cell lines were employed and exposed to various concentrations of bazedoxifene, paclitaxel, gemcitabine, and their combinations for cell viability, colony formation, and wound healing assays to demonstrate the antitumor effect of bazedoxifene with or without paclitaxel or gemcitabine.

Results

MTT cell viability, colony formation, and wound healing assays showed that bazedoxifene was capable of inhibiting cell viability, colony formation, and cell migration in a dose-dependent manner. In addition, bazedoxifene was capable of working with paclitaxel or gemcitabine synergistically to inhibit cell viability, colony formation, and cell migration.

Conclusion

This study demonstrated the potential antitumor effect of bazedoxifene and its ability to improve the treatment efficacy of paclitaxel and gemcitabine.

Abstract Image

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比达昔芬抑制人类非小细胞肺癌细胞的细胞活力、集落形成活性和细胞迁移，并提高紫杉醇和吉西他滨的治疗效果

背景巴唑昔芬是一种第三代选择性雌激素受体调节剂，它通过抑制 IL6 诱导的 GP130 同源二聚化来抑制 IL6/IL6R/GP130 信号通路。考虑到IL6/IL6R/GP130信号通路在肿瘤发生和转移中的重要作用，巴唑昔芬被认为具有抗肿瘤作用，这已在乳腺癌和胰腺癌中得到初步证实，但尚未在非小细胞肺癌（NSCLC）中进行研究。本研究旨在评估巴唑昔芬对 NSCLC 的抗肿瘤作用。方法采用 A549 和 H1299 NSCLC 细胞株，将其暴露于不同浓度的巴唑昔芬、紫杉醇、吉西他滨以及它们的复方制剂中，进行细胞活力、菌落形成和伤口愈合检测，以证明巴唑昔芬与紫杉醇或吉西他滨联合或不联合的抗肿瘤效果。结果 MTT 细胞活力、集落形成和伤口愈合试验表明，巴唑昔芬能以剂量依赖性方式抑制细胞活力、集落形成和细胞迁移。此外，巴唑昔芬还能与紫杉醇或吉西他滨协同抑制细胞活力、集落形成和细胞迁移。结论本研究证明了巴唑昔芬潜在的抗肿瘤作用及其改善紫杉醇和吉西他滨治疗效果的能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical Respiratory Journal 医学-呼吸系统

CiteScore

3.70

自引率

0.00%

发文量

104

审稿时长

>12 weeks

期刊介绍： Overview Effective with the 2016 volume, this journal will be published in an online-only format. Aims and Scope The Clinical Respiratory Journal (CRJ) provides a forum for clinical research in all areas of respiratory medicine from clinical lung disease to basic research relevant to the clinic. We publish original research, review articles, case studies, editorials and book reviews in all areas of clinical lung disease including: Asthma Allergy COPD Non-invasive ventilation Sleep related breathing disorders Interstitial lung diseases Lung cancer Clinical genetics Rhinitis Airway and lung infection Epidemiology Pediatrics CRJ provides a fast-track service for selected Phase II and Phase III trial studies. Keywords Clinical Respiratory Journal, respiratory, pulmonary, medicine, clinical, lung disease, Abstracting and Indexing Information Academic Search (EBSCO Publishing) Academic Search Alumni Edition (EBSCO Publishing) Embase (Elsevier) Health & Medical Collection (ProQuest) Health Research Premium Collection (ProQuest) HEED: Health Economic Evaluations Database (Wiley-Blackwell) Hospital Premium Collection (ProQuest) Journal Citation Reports/Science Edition (Clarivate Analytics) MEDLINE/PubMed (NLM) ProQuest Central (ProQuest) Science Citation Index Expanded (Clarivate Analytics) SCOPUS (Elsevier)

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