Transcription start site choice regulates HIV-1 RNA conformation and function

IF 6.1 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Current opinion in structural biology Pub Date : 2024-08-14 DOI:10.1016/j.sbi.2024.102896

Karin Musier-Forsyth , Alan Rein , Wei-Shau Hu

引用次数: 0

Abstract

HIV-1, the causative agent of AIDS, is a retrovirus that packages two copies of unspliced viral RNA as a dimer into newly budding virions. The unspliced viral RNA also serves as an mRNA template for translation of two polyproteins. Recent studies suggest that the fate of the viral RNA (genome or mRNA) is determined at the level of transcription. RNA polymerase II uses heterogeneous transcription start sites to generate major transcripts that differ in only two guanosines at the 5ʹ end. Remarkably, this two-nucleotide difference is sufficient to alter the structure of the 5ʹ-untranslated region and generate two pools of RNA with distinct functions. The presence of both RNA species is needed for optimal viral replication and fitness.

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转录起始位点的选择调节 HIV-1 RNA 的构象和功能。

艾滋病的病原体 HIV-1 是一种逆转录病毒，它将两份未拼接的病毒 RNA 作为二聚体打包到新萌发的病毒中。未拼接的病毒 RNA 也是翻译两种多聚蛋白的 mRNA 模板。最新研究表明，病毒 RNA（基因组或 mRNA）的命运取决于转录水平。RNA 聚合酶 II 利用异质转录起始位点产生主要转录本，这些转录本的 5' 端只有两个鸟苷酸不同。值得注意的是，这两个核苷酸的差异足以改变 5'-非翻译区的结构，并产生两个具有不同功能的 RNA 池。这两种 RNA 的存在对病毒的最佳复制和适应性都是必需的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current opinion in structural biology 生物-生化与分子生物学

CiteScore

12.20

自引率

2.90%

发文量

179

审稿时长

6-12 weeks

期刊介绍： Current Opinion in Structural Biology (COSB) aims to stimulate scientifically grounded, interdisciplinary, multi-scale debate and exchange of ideas. It contains polished, concise and timely reviews and opinions, with particular emphasis on those articles published in the past two years. In addition to describing recent trends, the authors are encouraged to give their subjective opinion of the topics discussed. In COSB, we help the reader by providing in a systematic manner: 1. The views of experts on current advances in their field in a clear and readable form. 2. Evaluations of the most interesting papers, annotated by experts, from the great wealth of original publications. [...] The subject of Structural Biology is divided into twelve themed sections, each of which is reviewed once a year. Each issue contains two sections, and the amount of space devoted to each section is related to its importance. -Folding and Binding- Nucleic acids and their protein complexes- Macromolecular Machines- Theory and Simulation- Sequences and Topology- New constructs and expression of proteins- Membranes- Engineering and Design- Carbohydrate-protein interactions and glycosylation- Biophysical and molecular biological methods- Multi-protein assemblies in signalling- Catalysis and Regulation