Molecular Survey of Tick-Borne Haemoparasites of Dogs by Multiplex Polymerase Chain Reaction from Punjab, India.

IF 1.2 3区农林科学 Q4 PARASITOLOGY

Acta Parasitologica Pub Date : 2024-09-01 Epub Date: 2024-08-16 DOI:10.1007/s11686-024-00856-5

Harkirat Singh, M Padmaja, Aparna M Thomas, Harsh Panwar, Shaikh I Nasrul, Jyoti, Nirbhay K Singh

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引用次数: 0

Abstract

Purpose: Tick-transmitted parasites as Babesia gibsoni, Babesia vogeli, Ehrlichia canis, and Hepatozoon canis are major health concern for dogs. Owing to prevalence and infection severity, there is need of sensitive, specific, and affordable test for their simultaneous detection.

Methods: Prevalence of B. gibsoni, B. vogeli, E. canis, and H. canis infections was assessed on 719 blood samples by microscopy and multiplex PCR assay targeting 18S rRNA (B. gibsoni & H. canis), ITS1 & 5.8S rRNA (B. vogeli) and VirB9 gene (E. canis). An internal control (canine-actin) was also included to increase the accuracy of assay and effect of associated risk factors with disease prevalence was also studied.

Results: Microscopic prevalence of B. gibsoni, B. vogeli, E. canis and H. canis was 5.0%, 0.1%, 1.4% and 1.0%, respectively, whereas with multiplex PCR assay, the corresponding values were 8.9%, 1.1%, 2.6% and 5.1% besides concurrent infections of B. gibsoni & H. canis (0.4%), B. gibsoni & E. canis (0.4%), E. canis & H. canis (0.3%) and B. gibsoni & B. vogeli (0.1%). Analytical sensitivity of developed assay was 0.1pg (B. gibsoni & H. canis), 0.01pg (B. vogeli), and 1.0pg (E. canis). A ″fair″ (B. vogeli & H. canis) to ″substantial″ (B. gibsoni & E. canis) agreement between two tests was observed with data as statistically significant. Breed, sex and location were significantly associated with B. gibsoni infection.

Conclusion: The developed multiplex PCR assay offers a potential solution to detect these pathogens simultaneously, aiding in timely diagnosis and effective disease management in suspected dogs.

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通过多重聚合酶链式反应对印度旁遮普邦犬蜱血寄生虫的分子调查。

目的：由蜱虫传播的寄生虫，如吉布逊蜱、沃格利蜱、犬艾氏杆菌和犬肝吸虫，是狗的主要健康问题。由于这些寄生虫的流行和感染的严重程度，需要一种灵敏、特异且经济实惠的检测方法来同时检测这些寄生虫：方法：通过显微镜和针对 18S rRNA（吉布索尼犬和犬黑线虫）、ITS1 和 5.8S rRNA（吉布索尼犬和犬黑线虫）以及 VirB9 基因（犬黑线虫）的多重 PCR 检测，评估了 719 份血液样本中吉布索尼犬、犬黑线虫、犬黑线虫和犬黑线虫的感染率。为了提高检测的准确性，还加入了内部对照（犬肌动蛋白），并研究了相关风险因素对疾病流行的影响：结果：在显微镜下，吉布森氏杆菌、沃格利氏杆菌、犬大肠杆菌和犬小肠杆菌的患病率分别为 5.0%、0.1%、1.4% 和 1.0%，而在多重 PCR 检测中，相应的患病率分别为 8.9%、1.1%、2.0%。9%、1.1%、2.6% 和 5.1%，此外还有吉布森氏杆菌和犬细小病毒（0.4%）、吉布森氏杆菌和犬细小病毒（0.4%）、犬细小病毒和犬细小病毒（0.3%）以及吉布森氏杆菌和沃格利氏杆菌（0.1%）的并发感染。所开发的检测方法的分析灵敏度分别为 0.1pg （吉布森氏杆菌和犬白喉杆菌）、0.01pg （伏格利氏杆菌）和 1.0pg （犬白喉杆菌）。两次测试的结果 "一般"（B. vogeli 和 H. canis）到 "相当"（B. gibsoni 和 E. canis）一致，数据具有统计学意义。犬种、性别和地点与吉布森氏杆菌感染有明显相关性：结论：所开发的多重 PCR 检测方法为同时检测这些病原体提供了一种潜在的解决方案，有助于对可疑犬只进行及时诊断和有效的疾病管理。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Parasitologica 医学-寄生虫学

CiteScore

3.10

自引率

6.70%

发文量

149

审稿时长

6-12 weeks

期刊介绍： Acta Parasitologica is an international journal covering the latest advances in the subject. Acta Parasitologica publishes original papers on all aspects of parasitology and host-parasite relationships, including the latest discoveries in biochemical and molecular biology of parasites, their physiology, morphology, taxonomy and ecology, as well as original research papers on immunology, pathology, and epidemiology of parasitic diseases in the context of medical, veterinary and biological sciences. The journal also publishes short research notes, invited review articles, book reviews. The journal was founded in 1953 as "Acta Parasitologica Polonica" by the Polish Parasitological Society and since 1954 has been published by W. Stefanski Institute of Parasitology of the Polish Academy of Sciences in Warsaw. Since 1992 in has appeared as Acta Parasitologica in four issues per year.