Strategic approaches for designing yeast strains as protein secretion and display platforms.

IF 8.1 2区工程技术 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Critical Reviews in Biotechnology Pub Date : 2024-08-13 DOI:10.1080/07388551.2024.2385996

Luping Xu, Xingjian Bai, Eun Joong Oh

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引用次数: 0

Abstract

Yeast has been established as a versatile platform for expressing functional molecules, owing to its well-characterized biology and extensive genetic modification tools. Compared to prokaryotic systems, yeast possesses advanced cellular mechanisms that ensure accurate protein folding and post-translational modifications. These capabilities are particularly advantageous for the expression of human-derived functional proteins. However, designing yeast strains as an expression platform for proteins requires the integration of molecular and cellular functions. By delving into the complexities of yeast-based expression systems, this review aims to empower researchers with the knowledge to fully exploit yeast as a functional platform to produce a diverse range of proteins. This review includes an exploration of the host strains, gene cassette structures, as well as considerations for maximizing the efficiency of the expression system. Through this in-depth analysis, the review anticipates stimulating further innovation in the field of yeast biotechnology and protein engineering.

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设计酵母菌株作为蛋白质分泌和展示平台的战略方法。

酵母具有良好的生物学特性和广泛的基因修饰工具，已被确立为表达功能分子的多功能平台。与原核系统相比，酵母拥有先进的细胞机制，可确保蛋白质的精确折叠和翻译后修饰。这些能力对于表达源自人类的功能性蛋白质尤为有利。然而，将酵母菌株设计为蛋白质表达平台需要整合分子和细胞功能。本综述深入探讨了基于酵母的表达系统的复杂性，旨在让研究人员掌握相关知识，充分利用酵母作为功能平台来生产各种蛋白质。这篇综述探讨了宿主菌株、基因盒结构以及最大限度提高表达系统效率的注意事项。通过深入分析，本综述预计将激励酵母生物技术和蛋白质工程领域的进一步创新。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Critical Reviews in Biotechnology 工程技术-生物工程与应用微生物

CiteScore

20.80

自引率

1.10%

发文量

审稿时长

4.8 months

期刊介绍： Biotechnological techniques, from fermentation to genetic manipulation, have become increasingly relevant to the food and beverage, fuel production, chemical and pharmaceutical, and waste management industries. Consequently, academic as well as industrial institutions need to keep abreast of the concepts, data, and methodologies evolved by continuing research. This journal provides a forum of critical evaluation of recent and current publications and, periodically, for state-of-the-art reports from various geographic areas around the world. Contributing authors are recognized experts in their fields, and each article is reviewed by an objective expert to ensure accuracy and objectivity of the presentation.