Advances in point-of-care and molecular techniques to detect waterborne pathogens

IF 10.4 1区 工程技术 Q1 ENGINEERING, CHEMICAL
Meysam Khodaparast, Dave Sharley, Stephen Marshall, Travis Beddoe
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引用次数: 0

Abstract

Rapid and precise detection of waterborne pathogens is critical for effective public health management and environmental safety. Traditional methods for water quality monitoring, considered the “Gold Standard,” are time-consuming, costly, and rely on centralized laboratories and expertise. These methods are impractical for on-site, real-time monitoring, and requiring further improvements. This review explores various waterborne pathogens and cutting-edge molecular detection techniques. It highlights the growing importance of point-of-care and point-of-application methods to expedite results and improve health risk management. Loop-mediated isothermal amplification emerges as a reliable, rapid, and accessible tool in the realms of on-site diagnostics and surveillance. Moreover, the review emphasizes the crucial role of water sample preparation and in-field nucleic acid isolation in augmenting pathogen detection to enable precise assessments of water quality. The advancement of these techniques will guarantee access to safe water and improve the management of waterborne diseases.

Abstract Image

检测水传播病原体的护理点和分子技术的进步
快速、精确地检测水传播病原体对于有效的公共卫生管理和环境安全至关重要。被视为 "黄金标准 "的传统水质监测方法耗时长、成本高,而且依赖于集中实验室和专业知识。这些方法不适合现场实时监测,需要进一步改进。本综述探讨了各种水传播病原体和最先进的分子检测技术。它强调了护理点和应用点方法在加快检测结果和改善健康风险管理方面日益增长的重要性。在现场诊断和监测领域,环介导等温扩增技术是一种可靠、快速、易用的工具。此外,综述还强调了水样制备和现场核酸分离在增强病原体检测以实现水质精确评估方面的关键作用。这些技术的进步将保证人们能够获得安全的饮用水,并改善水传播疾病的管理。
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来源期刊
npj Clean Water
npj Clean Water Environmental Science-Water Science and Technology
CiteScore
15.30
自引率
2.60%
发文量
61
审稿时长
5 weeks
期刊介绍: npj Clean Water publishes high-quality papers that report cutting-edge science, technology, applications, policies, and societal issues contributing to a more sustainable supply of clean water. The journal's publications may also support and accelerate the achievement of Sustainable Development Goal 6, which focuses on clean water and sanitation.
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