Discovery of a new bacterium, Microbacterium betulae sp. nov., in birch wood associated with hypersensitivity pneumonitis in woodworkers

IF 3.6 4区生物学 Q2 ENVIRONMENTAL SCIENCES

Environmental Microbiology Reports Pub Date : 2024-08-12 DOI:10.1111/1758-2229.13311

Mariola Paściak, Krzysztof J. Pawlik, Dariusz Martynowski, Łukasz Łaczmański, Jarosław Ciekot, Bogumiła Szponar, Angelina Wójcik-Fatla, Barbara Mackiewicz, Ewelina Farian, Grażyna Cholewa, Alicja Cholewa, Jacek Dutkiewicz

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Abstract

A Gram-positive, aerobic, rod-shaped mesophilic bacterium was isolated from birch wood, referred to as the AB strain. Allergological tests suggest that this strain may cause allergic alveolitis in sawmill workers. Employing a polyphasic taxonomic approach, the AB strain's 16S rRNA gene sequence showed high similarity to Microbacterium barkeri and M. oryzae, with 97.25% and 96.91%, respectively, a finding supported by rpoB and gyrB sequence analysis. Further genome sequence comparison with the closely related M. barkeri type strain indicated a digital DNA–DNA hybridization value of 25.5% and an average nucleotide identity of 82.52%. The AB strain's cell wall peptidoglycan contains ornithine, and its polar lipids comprise diphosphatidylglycerol, phosphatidylglycerol, and unidentified glycolipids. Its major fatty acids include anteiso C15:0, anteiso C17:0, and iso C16:0, while MK-10 is its predominant respiratory quinone. Comprehensive analysis through 16S rRNA, whole-genome sequencing, phenotyping, chemotaxonomy, and MALDI-TOF MS profiling indicates that the AB strain represents a new species within the Microbacterium genus. It has been proposed to name this species Microbacterium betulae sp. nov., with AB^T (PCM 3040^T = CEST 30706^T) designated as the type strain.

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在与木工超敏性肺炎有关的桦木中发现一种新细菌--betulae sp.

从桦木中分离出一种革兰氏阳性、需氧、棒状的嗜中性细菌，称为 AB 菌株。过敏试验表明，这种菌株可能会导致锯木厂工人患上过敏性肺泡炎。采用多相分类法，AB 菌株的 16S rRNA 基因序列与树皮微杆菌和 M. oryzae 的相似度很高，分别为 97.25% 和 96.91%，rpoB 和 gyrB 序列分析也证实了这一结论。与密切相关的 M. barkeri 型菌株进行的进一步基因组序列比较表明，其数字 DNA-DNA 杂交值为 25.5%，平均核苷酸同一性为 82.52%。AB 株的细胞壁肽聚糖含有鸟氨酸，极性脂质包括二磷脂酰甘油、磷脂酰甘油和不明糖脂。其主要脂肪酸包括反式 C15:0、反式 C17:0 和异式 C16:0，而 MK-10 是其主要的呼吸醌。通过 16S rRNA、全基因组测序、表型分析、化学分类学和 MALDI-TOF MS 图谱进行的综合分析表明，AB 菌株代表了微杆菌属中的一个新物种。已建议将该物种命名为 betulae 微杆菌新种（Microbacterium betulae sp.

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来源期刊

Environmental Microbiology Reports ENVIRONMENTAL SCIENCES-MICROBIOLOGY

CiteScore

6.00

自引率

3.00%

发文量

审稿时长

3.0 months

期刊介绍： The journal is identical in scope to Environmental Microbiology, shares the same editorial team and submission site, and will apply the same high level acceptance criteria. The two journals will be mutually supportive and evolve side-by-side. Environmental Microbiology Reports provides a high profile vehicle for publication of the most innovative, original and rigorous research in the field. The scope of the Journal encompasses the diversity of current research on microbial processes in the environment, microbial communities, interactions and evolution and includes, but is not limited to, the following: the structure, activities and communal behaviour of microbial communities microbial community genetics and evolutionary processes microbial symbioses, microbial interactions and interactions with plants, animals and abiotic factors microbes in the tree of life, microbial diversification and evolution population biology and clonal structure microbial metabolic and structural diversity microbial physiology, growth and survival microbes and surfaces, adhesion and biofouling responses to environmental signals and stress factors modelling and theory development pollution microbiology extremophiles and life in extreme and unusual little-explored habitats element cycles and biogeochemical processes, primary and secondary production microbes in a changing world, microbially-influenced global changes evolution and diversity of archaeal and bacterial viruses new technological developments in microbial ecology and evolution, in particular for the study of activities of microbial communities, non-culturable microorganisms and emerging pathogens.