Macaque liver substrate for evaluating dense fine speckled-like patterns on HEp2010 cells

IF 3.6 3区医学 Q1 PATHOLOGY

Pathology Pub Date : 2024-08-03 DOI:10.1016/j.pathol.2024.05.011

Anthea Anantharajah, Roger A. Silvestrini, David Campbell, Suzanne Culican, Adrian Y.S. Lee, Ming Wei Lin

引用次数: 0

Abstract

Antinuclear antibody (ANA) detection by indirect immunofluorescence (IIF) is instrumental in the evaluation of systemic autoimmune diseases (SAD). The dense fine speckled (DFS) ANA staining predominantly associates with anti-DFS70, an autoantibody that is thought to exclude the presence of SAD. However, the DFS pattern may mask the presence of other ANA patterns that may be clinically relevant. Our laboratory uses the HEp2010 substrate which contains both HEp2 and liver substates. The aim of this study was to determine whether negative liver nucleus immunofluorescence could exclude the presence of antibodies to extractable nuclear antigens (ENA) in sera with DFS-like patterns.

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用于评估 HEp2010 细胞上密集细小斑点状图案的猕猴肝脏基质

通过间接免疫荧光（IIF）检测抗核抗体（ANA）有助于评估全身性自身免疫性疾病（SAD）。致密细斑点（DFS）ANA 染色主要与抗DFS70 相关联，这种自身抗体被认为可以排除 SAD 的存在。然而，DFS 模式可能会掩盖其他可能与临床相关的 ANA 模式。我们实验室使用的 HEp2010 底物包含 HEp2 和肝脏底物。本研究的目的是确定阴性肝核免疫荧光能否排除 DFS 样本血清中存在的可提取核抗原（ENA）抗体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pathology 医学-病理学

CiteScore

6.50

自引率

2.20%

发文量

459

审稿时长

54 days

期刊介绍： Published by Elsevier from 2016 Pathology is the official journal of the Royal College of Pathologists of Australasia (RCPA). It is committed to publishing peer-reviewed, original articles related to the science of pathology in its broadest sense, including anatomical pathology, chemical pathology and biochemistry, cytopathology, experimental pathology, forensic pathology and morbid anatomy, genetics, haematology, immunology and immunopathology, microbiology and molecular pathology.