Highly extensible physically crosslinked hydrogels for high-speed 3D bioprinting

Ye Eun Song, Noah Eckman, Samya Sen, Olivia M. Saouaf, Eric A. Appel
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Abstract

Hydrogels have emerged as promising materials for bioprinting and many other biomedical applications due to their high degree of biocompatibility and ability to support and/or modulate cell viability and function. Yet, many hydrogel bioinks have suffered from low efficiency due to limitations on accessible printing speeds, often limiting cell viability and/or the constructs which can be generated. In this study, we report a highly extensible bioink system created by modulating the rheology of physically crosslinked hydrogels comprising hydrophobically modified cellulosic biopolymers and additives such as surfactants or cyclodextrins. We demonstrate that these hydrogel materials are highly shear-thinning with broadly tunable viscoelasticity and stress-relaxation behaviors through simple modulation of the composition of the additives. Rheological experiments demonstrate that increasing concentration of rheology-modifying additives yields hydrogel materials exhibiting extensional strain-to-break values up to 2000%. We demonstrate the potential of these hydrogels for use as bioinks by evaluating the relationship between extensibility and printability, demonstrating that greater hydrogel extensibility enables faster print speeds and smaller print features. Our findings suggest that optimizing hydrogel extensibility can enhance high-speed 3D bioprinting capabilities.
用于高速三维生物打印的高延展性物理交联水凝胶
水凝胶因其高度的生物相容性以及支持和/或调节细胞活力和功能的能力,已成为生物打印和许多其他生物医学应用的理想材料。然而,许多水凝胶生物墨水由于可打印速度的限制而效率低下,这往往限制了细胞存活率和/或可生成的构建体。在本研究中,我们报告了一种通过调节物理交联水凝胶的流变性而产生的高延展性生物墨水系统,这种水凝胶由疏水改性纤维素生物聚合物和添加剂(如表面活性剂或环糊精)组成。我们的研究表明,这些水凝胶材料具有高度剪切稀化特性,通过简单地调节添加剂的成分就能实现广泛的粘弹性和应力松弛行为。流变学实验证明,增加流变修饰添加剂的浓度可使水凝胶材料显示出高达 2000% 的延伸应变至断裂值。我们通过评估延展性和可印刷性之间的关系,证明了这些水凝胶作为生物墨水使用的潜力,并证明了更大的水凝胶延展性可实现更快的印刷速度和更小的印刷特征。我们的研究结果表明,优化水凝胶的延展性可以提高高速三维生物打印能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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