Insights into free radicals scavenging, α-Amylase inhibition, cytotoxic and antifibrotic activities unveiled by Peganum harmala extracts

2区 医学 Q1 Medicine
Nidal Jaradat, Mohammed Hawash, Majid Sharifi-Rad, Ali Shakhshir, Shorooq Sobuh, Fatima Hussein, Linda Issa, Sondos Hamamrhe, Eman Al-Sheikh, Alaa Naser Ibrahim
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引用次数: 0

Abstract

Peganum harmala L. is used in traditional medicine to treat several health ailments. Hence, the present work aimed to investigate the DPPH free radical scavenging, α-amylase, cytotoxic, and antifibrotic effects of the hydrophilic extract and fixed oil obtained from P. harmala seeds. The hydrophilic extract and fixed oil of P. harmala were assessed for their abilities to scavenge DPPH free radicals and inhibit α-amylase using reference bioassays. The cytotoxicity was assessed on several cancer and normal cell lines, including B16F1, Caco-2, COLO205, HeLa, Hep 3B and Hep G2, MCF-7, and HEK-293 T cells. The MTS assay was used to evaluate the antifibrotic capabilities utilizing the human hepatic stellate (LX-2) cell line. P. harmala plant fixed oil has potent DPPH free radical scavenging activity with an IC50 dose of 79.43 ± 0.08 µg/ml. Besides, the hydrophilic extract has a poor anti-α-amylase effect compared with the antidiabetic drug Acarbose, with IC50 doses of 398 ± 0.59 and 25.11 ± 1.22 µg/ml, respectively. In addition, the growth of MCF-7, Hep3B, HepG2, HeLa, COLO205, CaCo2, B16F1, and HeK293t was inhibited by P. harmala hydrophilic extract with IC50 doses of 121.34 ± 1.71, 268.3 ± 0.75, 297.20 ± 1.00, 155.60 ± 1.14, 150.01 ± 0.51, 308.35 ± 0.53, 597.93 ± 1.36, and 5.38 ± 0.99 µg/ml, respectively. In addition, at 1000 µg/ml, 5-Fluorouracil reduced fibrosis cells by 0.089%, while the hydrophilic extract decreased the number of LX-2 cells by 5.81%. P. harmala plant-fixed oil exhibits potential antioxidant properties. While the hydrophilic extract showed limited effectiveness as an anti-α-amylase agent and demonstrated notable cytotoxic effects against various tested cancer cell lines. Furthermore, this extract significantly reduces the number of LX-2 fibrotic cells. These findings emphasize the therapeutic potential of these products in managing various health disorders and warrant further investigation into their mechanisms of action and clinical applications.
揭示牛膝提取物清除自由基、抑制α-淀粉酶、细胞毒性和抗纤维化活性的奥秘
Peganum harmala L.在传统医学中被用于治疗多种疾病。因此,本研究旨在调查从 P. harmala 种子中提取的亲水提取物和固定油的 DPPH 自由基清除、α-淀粉酶、细胞毒性和抗纤维化作用。采用参考生物测定法评估了哈马拉种子亲水提取物和固定油清除 DPPH 自由基和抑制 α 淀粉酶的能力。对几种癌症和正常细胞系进行了细胞毒性评估,包括 B16F1、Caco-2、COLO205、HeLa、Hep 3B 和 Hep G2、MCF-7 和 HEK-293 T 细胞。MTS 试验用于评估人肝星状细胞(LX-2)的抗纤维化能力。哈马拉植物固定油具有强效的 DPPH 自由基清除活性,其 IC50 剂量为 79.43 ± 0.08 µg/ml。此外,与抗糖尿病药物阿卡波糖相比,亲水提取物的抗α-淀粉酶效果较差,IC50剂量分别为 398 ± 0.59 和 25.11 ± 1.22 µg/ml。此外,哈马拉亲水提取物还能抑制 MCF-7、Hep3B、HepG2、HeLa、COLO205、CaCo2、B16F1 和 HeK293t 的生长,IC50 剂量分别为 121.34 ± 1.71、268.3 ± 0.75、297.20 ± 1.00、155.60 ± 1.14、150.01 ± 0.51、308.35 ± 0.53、597.93 ± 1.36 和 5.38 ± 0.99 µg/ml。此外,在 1000 µg/ml 的浓度下,5-氟尿嘧啶可使纤维化细胞数量减少 0.089%,而亲水提取物可使 LX-2 细胞数量减少 5.81%。哈马拉植物固定油具有潜在的抗氧化特性。亲水萃取物作为一种抗α-淀粉酶制剂显示出有限的有效性,并对各种测试的癌细胞株显示出显著的细胞毒性作用。此外,这种提取物还能明显减少 LX-2 纤维化细胞的数量。这些发现强调了这些产品在治疗各种健康疾病方面的潜力,值得进一步研究其作用机制和临床应用。
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来源期刊
BMC Complementary and Alternative Medicine
BMC Complementary and Alternative Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
7.00
自引率
0.00%
发文量
0
审稿时长
3 months
期刊介绍: BMC Complementary Medicine and Therapies is an open access journal publishing original peer-reviewed research articles on interventions and resources that complement or replace conventional therapies, with a specific emphasis on research that explores the biological mechanisms of action, as well as their efficacy, safety, costs, patterns of use and/or implementation.
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