QuEChERS Method for Simultaneous Analysis of Tetracyclines from Chicken by HPLC–DAD

IF 2.6 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Analytical Methods Pub Date : 2024-08-12 DOI:10.1007/s12161-024-02663-0

João Victor Morais Gurgel, Luciane Effting, Diego Galvan, Carlos Eduardo Domingues Nazário

{"title":"QuEChERS Method for Simultaneous Analysis of Tetracyclines from Chicken by HPLC–DAD","authors":"João Victor Morais Gurgel, Luciane Effting, Diego Galvan, Carlos Eduardo Domingues Nazário","doi":"10.1007/s12161-024-02663-0","DOIUrl":null,"url":null,"abstract":"<div><p>Tetracyclines (TCs) are among the most widely used antibiotics worldwide for treating bacterial infections (both Gram-positive and Gram-negative) in humans and animals. In Brazil, chicken is one of the most produced and traded meats, with TCs being the primary antibiotics used in its production. This study applied two experimental designs to optimize the QuEChERS method for simultaneous quantitative analysis by HPLC–DAD of three TCs (tetracycline, chlortetracycline, and oxytetracycline) in chicken. The optimized conditions of the QuEChERS method for the preconcentration of TCs were 500 mg of sample, 1000 mg of Na<sub>2</sub>SO<sub>4</sub>, 700 mg of NaCl, 100 mg C18, 100 mg of PSA in 10 mL of the extracting solvent, vortex for 1 min, and centrifugation 4000 rpm for 5 min with a total run time of 10 min. LOQ values were lower than the MRL for all antibiotics established by ANVISA, precision in intra-day and inter-day ranged from 0.1 to 5.0%, and recovery ranged from 80 to 101%, greatly improving the work efficiency. The analytical method was applied to four commercial chicken breast samples and other proteins, one for beef and one for pork, of which no residues were found. The “GREEnness” of the optimized method demonstrated that the proposed approach made it possible to develop a “greener” method than traditional methods.</p></div>","PeriodicalId":561,"journal":{"name":"Food Analytical Methods","volume":"17 9","pages":"1292 - 1301"},"PeriodicalIF":2.6000,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Analytical Methods","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s12161-024-02663-0","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Tetracyclines (TCs) are among the most widely used antibiotics worldwide for treating bacterial infections (both Gram-positive and Gram-negative) in humans and animals. In Brazil, chicken is one of the most produced and traded meats, with TCs being the primary antibiotics used in its production. This study applied two experimental designs to optimize the QuEChERS method for simultaneous quantitative analysis by HPLC–DAD of three TCs (tetracycline, chlortetracycline, and oxytetracycline) in chicken. The optimized conditions of the QuEChERS method for the preconcentration of TCs were 500 mg of sample, 1000 mg of Na₂SO₄, 700 mg of NaCl, 100 mg C18, 100 mg of PSA in 10 mL of the extracting solvent, vortex for 1 min, and centrifugation 4000 rpm for 5 min with a total run time of 10 min. LOQ values were lower than the MRL for all antibiotics established by ANVISA, precision in intra-day and inter-day ranged from 0.1 to 5.0%, and recovery ranged from 80 to 101%, greatly improving the work efficiency. The analytical method was applied to four commercial chicken breast samples and other proteins, one for beef and one for pork, of which no residues were found. The “GREEnness” of the optimized method demonstrated that the proposed approach made it possible to develop a “greener” method than traditional methods.

Abstract Image

查看原文本刊更多论文

用 HPLC-DAD 同时分析鸡肉中四环素的 QuEChERS 方法

四环素（TC）是全球使用最广泛的抗生素之一，用于治疗人类和动物的细菌感染（包括革兰氏阳性和革兰氏阴性）。在巴西，鸡肉是生产和交易量最大的肉类之一，而 TCs 是其生产过程中使用的主要抗生素。本研究采用了两种实验设计来优化 QuEChERS 方法，以便通过 HPLC-DAD 同时定量分析鸡肉中的三种三环素（四环素、金霉素和土霉素）。QuEChERS方法预富集TCs的优化条件为：500 mg样品、1000 mg Na2SO4、700 mg NaCl、100 mg C18、100 mg PSA加入10 mL提取溶剂中，涡旋1 min，4000 rpm离心5 min，总运行时间为10 min。所有抗生素的最低检测限（LOQ）均低于ANVISA规定的最高残留限量（MRL），日内和日间精密度为0.1%～5.0%，回收率为80%～101%，大大提高了工作效率。该分析方法应用于四种商品鸡胸肉样品和其他蛋白质样品，其中牛肉和猪肉各一种，均未发现残留。优化方法的 "GREEnness "表明，与传统方法相比，所提出的方法使开发一种 "更环保 "的方法成为可能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.