Identification and quantitation of regenerated casein fibers in combination fabric based on lateral flow immunoassay

IF 2.2 4区化学 Q2 Engineering

Chemical Papers Pub Date : 2024-08-05 DOI:10.1007/s11696-024-03628-7

Ronghu Feng, Shiwei Zhang, Ying He, Huiling Wu, Jiping Guo, Yingchun Zhou, Xintian Lai

{"title":"Identification and quantitation of regenerated casein fibers in combination fabric based on lateral flow immunoassay","authors":"Ronghu Feng, Shiwei Zhang, Ying He, Huiling Wu, Jiping Guo, Yingchun Zhou, Xintian Lai","doi":"10.1007/s11696-024-03628-7","DOIUrl":null,"url":null,"abstract":"<div><p>Regenerated protein fibers with different biogenetic derivations greatly vary in cost. However, finding a proper method for identifying and quantitating regenerated protein fibers is difficult. This study developed a rapid, inexpensive, convenient, and accurate qualitative immunochromatography method to detect regenerated casein fiber (RCF) in textiles. The RCF was characterized by electrospray ionization/time of flight mass spectrometry. Peptide haptens were designed based on an inert, conserved, and immunogenic sequence of regenerated casein. Monoclonal antibodies were prepared from the haptens, and a colloidal gold immunochromatography assay (GICA) was applied for the detection. A limit of detection of 0.1% RCF in textiles was achieved with a total analysis time of only 15 min using an extraction solution of 7 M urea containing 0.1% dithiothreitol and treatment for 10 min in boiling water. Quantitative assay results were obtained by using a GICA reader with the recovery rates of 110 to 130% and relative standard deviations of ≤ 20%.</p></div>","PeriodicalId":513,"journal":{"name":"Chemical Papers","volume":"78 13","pages":"7643 - 7650"},"PeriodicalIF":2.2000,"publicationDate":"2024-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Papers","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s11696-024-03628-7","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"Engineering","Score":null,"Total":0}

引用次数: 0

Abstract

Regenerated protein fibers with different biogenetic derivations greatly vary in cost. However, finding a proper method for identifying and quantitating regenerated protein fibers is difficult. This study developed a rapid, inexpensive, convenient, and accurate qualitative immunochromatography method to detect regenerated casein fiber (RCF) in textiles. The RCF was characterized by electrospray ionization/time of flight mass spectrometry. Peptide haptens were designed based on an inert, conserved, and immunogenic sequence of regenerated casein. Monoclonal antibodies were prepared from the haptens, and a colloidal gold immunochromatography assay (GICA) was applied for the detection. A limit of detection of 0.1% RCF in textiles was achieved with a total analysis time of only 15 min using an extraction solution of 7 M urea containing 0.1% dithiothreitol and treatment for 10 min in boiling water. Quantitative assay results were obtained by using a GICA reader with the recovery rates of 110 to 130% and relative standard deviations of ≤ 20%.

Abstract Image

查看原文本刊更多论文

基于横向流动免疫测定法的组合织物中再生酪蛋白纤维的鉴定与定量

不同生物基因衍生的再生蛋白质纤维成本差异很大。然而，要找到一种合适的方法来鉴定和量化再生蛋白纤维却很困难。本研究开发了一种快速、廉价、方便、准确的免疫层析定性方法来检测纺织品中的再生酪蛋白纤维（RCF）。电喷雾电离/飞行时间质谱法对再生酪蛋白纤维进行了表征。根据再生酪蛋白的惰性、保守和免疫原性序列设计了肽触素。利用肽键制备了单克隆抗体，并采用胶体金免疫层析法（GICA）进行检测。使用含有 0.1% 二硫苏糖醇的 7 M 尿素提取液，并在沸水中处理 10 分钟，纺织品中 RCF 的检测限为 0.1%，总分析时间仅为 15 分钟。使用 GICA 阅读器可获得定量分析结果，回收率为 110% 至 130%，相对标准偏差小于 20%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Chemical Papers Chemical Engineering-General Chemical Engineering

CiteScore

3.30

自引率

4.50%

发文量

590

期刊介绍： Chemical Papers is a peer-reviewed, international journal devoted to basic and applied chemical research. It has a broad scope covering the chemical sciences, but favors interdisciplinary research and studies that bring chemistry together with other disciplines.