Isolation of Amylase Producing Bacteria and Fungi from Kitchen Waste Water of Restaurants and Hostels in Ile-Ife, Osun State, Nigeria

Abstract

Organisms of industrial use are often isolated directly from the environment, most times from the soil, air and water. Amylase producing bacteria and fungi were obtained and isolated from kitchen waste water samples gotten from different hostels and restaurant across Ile-Ife, Osun State, Nigeria. The samples were first screened using pour plate method on nutrient agar and potato dextrose agar, then the isolates were further screened using other differential medium like starch agar. The isolates recovered were further characterized macroscopically, microscopically and biochemically to ascertain for amylase producing ability. Four bacterial and three fungal isolates were presumptively identified as; Bacillus macerans, Bacillus circulans, Bacillus brevis and Aspergillus niger, Aspergillus flavus , Aspergillus fumigatus.