NRP1 knockdown inhibits the invasion and migration of rhabdoid tumor of the kidney cells.

IF 1.5 3区医学 Q2 PEDIATRICS

Pediatric Surgery International Pub Date : 2024-08-12 DOI:10.1007/s00383-024-05808-8

Bin Yamaoka, Eri Nagasaki-Maeoka, Shota Uekusa, Eri Muto-Fujita, Naoko Abe, Kyoko Fujiwara, Tsugumichi Koshinaga, Shuichiro Uehara

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引用次数: 0

Abstract

Purpose: The aim of this study was to detect candidate oncogenes of rhabdoid tumor of the kidney (RTK) and evaluate their roles in RTK in vitro.

Methods: An integrated analysis of messenger RNA (mRNA) and microRNA (miRNA) sequencing was performed to determine the expression profile of exosome-derived miRNAs and mRNAs in human RTK-derived cell lines and a human embryonic renal cell line. A Gene Ontology enrichment analysis was performed to analyze the functional characteristics of differentially expressed mRNAs in RTK cells. Matrigel invasion and wound-healing assays were performed to evaluate the cell invasion and migration abilities.

Results: Forty mRNAs were highly expressed in RTK cells targeted by exosomal miRNAs, the expression of which was lower in RTK cells than in the controls. These mRNAs were primarily related to cell adhesion. Of these mRNAs, we selected neuropilin 1 (NRP1) as a candidate oncogene because its upregulated expression is associated with a poor prognosis of several types of tumors. RTK cells in which NRP1 had been knocked down exhibited decreased invasive and migratory abilities.

Conclusion: Our study indicates that NRP1 acts as an oncogene by promoting the invasion and migration of RTK cells and that it could serve as a therapeutic target.

Abstract Image

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敲除 NRP1 可抑制肾脏横纹肌瘤细胞的侵袭和迁移。

目的：本研究旨在检测肾横纹肌瘤（RTK）的候选癌基因，并评估它们在体外RTK中的作用：方法：对信使RNA（mRNA）和microRNA（miRNA）测序进行了综合分析，以确定外泌体衍生的miRNA和mRNA在人类RTK衍生细胞系和人类胚胎肾细胞系中的表达谱。研究人员还进行了基因本体富集分析，以分析 RTK 细胞中差异表达的 mRNA 的功能特征。进行了Matrigel侵袭和伤口愈合试验，以评估细胞的侵袭和迁移能力：结果：有40种mRNA在外泌体miRNA靶向的RTK细胞中高表达，而RTK细胞中这些mRNA的表达量低于对照组。这些 mRNA 主要与细胞粘附有关。在这些mRNA中，我们选择了神经蛋白1（NRP1）作为候选癌基因，因为它的表达上调与几种类型肿瘤的不良预后有关。NRP1被敲除的RTK细胞表现出侵袭和迁移能力下降：我们的研究表明，NRP1 通过促进 RTK 细胞的侵袭和迁移而成为一种癌基因，它可以作为一种治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pediatric Surgery International 医学-外科

CiteScore

3.00

自引率

5.60%

发文量

215

审稿时长

3-6 weeks

期刊介绍： Pediatric Surgery International is a journal devoted to the publication of new and important information from the entire spectrum of pediatric surgery. The major purpose of the journal is to promote postgraduate training and further education in the surgery of infants and children. The contents will include articles in clinical and experimental surgery, as well as related fields. One section of each issue is devoted to a special topic, with invited contributions from recognized authorities. Other sections will include: -Review articles- Original articles- Technical innovations- Letters to the editor